氨酸乙化调节H-NS的富含AT的DNA占有能力
Yabo Liu1, Mengqing Zhou1, Yifan Bu1
1State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China.
Nucleic acids research
|December 7, 2023
概括
细菌H-NS蛋白质乙化,特别是在K120中,减少了其DNA结合,增强了病毒性基因表达. 这项研究揭示了一种控制细菌基因沉默和毒性的新机制.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 遗传学 遗传学是一种遗传学.
背景情况:
- 基因组样核状结构蛋白 (H-NS) 对细菌基因组稳定性至关重要.
- H-NS使水平转移的基因沉默,包括III型和VI型分泌系统 (T3/T6SS).
- 相比于真核生物的基因组PTM,我们对 Prokaryotic H-NS的翻译后修饰 (PTM) 的理解很少.
研究的目的:
- 调查H-NS后翻译修饰 (PTMs) 的作用,特别是乙化,在调节基因沉默中的作用.
- 阐明H-NS乙化对DNA结合和细菌毒性的影响.
- 发现细菌基因调节的新机制.
主要方法:
- 液体染色学-并联质谱学 (LC-MS/MS) 分析,以确定H-NS上的乙氨酸位点.
- 对H-NSDNA结合亲和力和AT丰富DNA识别的分析.
- 在体内评估T3/T6SS表达和细菌毒性.
主要成果:
- 乙化H-NS减弱了它沉默水平转移基因的能力.
- 特定的乙氨酸位点,包括K120,对于H-NSDNA结合能力至关重要.
- 乙化K120降低了H-NSDNA结合亲和力,损害了AT丰富的DNA识别,并增强了T3/T6SS表达.
- 素乙化H-NS调节了细菌的毒性.
结论:
- H-NS 乙化,特别是在 K120 中,作为影响基因沉默和细菌毒性的调节机制.
- 这种乙化直接影响H-NS的DNA结合特性.
- 这些发现揭示了一种新的细菌策略,以抵消H-NS介导的异源性沉默.
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