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来自Lactobacillus helveticus的表面层蛋白质ATCC® 15009TM会影响肠道屏障的形态和功能

Paola Bendinelli¹, Ivano De Noni², Stefano Cattaneo²

¹Department of Biomedical Sciences for Health, University of Milan, Milan, Italy.

Tissue barriers

|December 7, 2023

概括

乳杆菌Helveticus的表面层蛋白质 (S层) 显示出有前途的副生物. 它们改善肠上皮质屏障功能,并抵消LPS诱导的炎症,与活体或非活化的细菌不同.

科学领域:

微生物学微生物学
胃肠病学胃肠病学
免疫学免疫学免疫学

背景情况:

偏微生物和后微生物为维持肠表皮屏障 (IEB) 健康提供了益生菌的替代品.
研究人员探讨了Lactobacillus helveticus表面层蛋白 (S层) 对IEB调制的影响.

研究的目的:

为了研究从Lb ATCC® 15009TM中S-layer作为一种偏微生物对IEB形态功能性质的影响.
为了在体外比较S层效应与活体或热不活化的Lb ATCC® 15009TM.

主要方法:

与Caco-2/HT-29细胞共同培养以建模肠道上皮屏障.
测量横体电阻 (TEER) 和细胞透性.
对于紧密结合蛋白 (Claudin-1) 的免疫光 (IF).
传输电子显微镜 (TEM) 用于结构分析.
评估性酸酶活性和介质蛋白-8的释放.

主要成果:

活体或无活体Lb ATCC® 15009TM损害了IEB功能和克劳丁-1分布.
在基底和LPS挑战条件下,S层改善了TEER和降低了透性.
由S层诱导的TJ重新排列和脱体形成,抵消LPS效应.
S层没有恢复IEB功能对抗促炎性细胞因子 (TNF-α + IFN-γ).

结论:

来自Lb ATCC® 15009TM的S层表现出一种类生物作用,增强了IEB的完整性.
S-layer 显示了对于胃肠道疾病和相关的肠外疾病的治疗潜力.

关键词:

克劳丁-1 是一种蛋白质.在LPS中使用LPS.在 S 层中,这就是为什么我们说desmosomes.免疫光效应免疫光效应肠道上皮质屏障肠道上皮质屏障类型类型类型类型类型类型紧密的结节紧密的结节传输电子显微镜的使用

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