一种来自Salmonella enterica血清型Enteritidis的ToxIN同源会损害细菌菌体感染的作用
John A McFarlane1, Eleanore G Hansen2, Estephany C Ortega2
1BioTechnology Institute, University of Minnesota, Saint Paul, MN 55108, USA.
Journal of applied microbiology
|December 7, 2023
概括
在食品传播的沙门氏菌和大肠杆菌中发现的ToxIN流产性感染系统,为实验室提供针对特定细菌菌的保护. 这个系统存在于各种沙门氏菌血清和致病性大肠杆菌菌株中.
科学领域:
- 细菌生物学的生物学
- 微生物遗传学微生物遗传学
- 食品安全微生物学 食品安全微生物学
背景情况:
- 菌体是感染细菌的病毒.
- 流产性感染系统是细菌对菌体的防御机制.
- 毒素系统在食物传播病原体中的作用尚未完全理解.
研究的目的:
- 调查食品传播的沙门氏菌中ToxIN流产性感染系统的存在.
- 评估ToxIN在保护特定菌体方面的有效性.
- 为了确定ToxIN在其他肠道细菌中的分布.
主要方法:
- BLASTN查询以识别沙门氏菌分离物中的毒素同类物.
- 克隆并将ToxIN同源转化为实验室菌株 (Salmonella typhimurium LT2,埃舍里希亚大肠杆菌 MG1655).
- 用特定的沙门氏菌和交叉属的菌体进行的菌体挑战测定.
- 对ToxIN等离子体存在的全基因组序列的分析.
主要成果:
- 在来自蛋爆发的食品传播的沙门氏菌肠炎中发现了一种ToxIN同源物.
- ToxIN对FelixO1,S16和LPST153菌体产生了耐药性,并减少了Sp6斑块的大小.
- 毒素IN提供了对感染沙门氏菌和大肠杆菌的菌体的跨属性保护.
- 在各种沙门氏菌血清,致病性大肠杆菌和其他肠道细菌中检测到ToxIN等离子体.
结论:
- 在实验室条件下,ToxIN系统赋予沙门氏菌和大肠杆菌对多种菌体的耐药性.
- 毒素IN存在于食物传播的病原体中,包括沙门氏菌和滋果毒性大肠杆菌.
- 这一发现对菌体治疗和食品安全策略有影响.
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