作为一种生物模拟性冷保护剂,聚氨酸-氨酸-协同氨酸-三氨酸糖酸) 是一种生物模拟性冷保护剂
Jin Kyung Park1, So-Jung Park1, Byeongmoon Jeong1
1Department of Chemistry and Nanoscience, Ewha Womans University, 52 Ewhayeodae-gil, Seodaemun-gu, Seoul 03760, Korea.
ACS applied materials & interfaces
|December 7, 2023
概括
我们合成了仿生抗聚合物 (PAT),模仿天然的糖蛋白. 优化的PAT显示出出色的冰再结晶抑制,冰核抑制和蛋白质冷保存,证明了冷保护应用的潜力.
科学领域:
- 生物材料科学 生物材料科学
- 聚合物化学 聚合物化学
- 生物化学 生物化学
背景情况:
- 自然防糖蛋白 (AFGPs) 通过形成冰的特性来防止生物体的寒冷损伤.
- 开发具有受控结构的合成类似物可以导致新型冷保护剂.
研究的目的:
- 为了合成和表征新的[l-Ala) -co-l-Thr糖酸盐] (PATs) 作为生物模拟抗糖蛋白类型.
- 评估PATs的冰再结晶抑制 (IRI),冰核化抑制 (INI),动态冰塑造 (DIS),热歇斯底里斯 (TH) 和蛋白质冷保存活动.
主要方法:
- 合成具有固定的分子量 (10-12 kDa) 和Ala/Thr比率 (2:1) 的PATs,可变的Ala含量和化.
- 评估了IRI,INI,DIS和TH活动.
- 通过冷解周期进行评估的蛋白质 (乳酸脱酶,LDH) 稳定.
主要成果:
- 含有较高l-Ala含量的PAT显示出增强的IRI和INI.
- 高化与观察到的动态冰形成 (DIS) 活动相关.
- 除了PATs-1之外,PATs有效地稳定了LDH,通过15个结解周期.
结论:
- 通过控制l-Ala含量和化,生物仿真PATs被成功合成.
- 具有65-70%l-Ala和12-19%化的PAT表现出显著的冷活性.
- 这些优化的PAT显示了蛋白质冷保护的前景,特别是对于LDH.
相关概念视频
01:44What are Carbohydrates?
Overview
02:22Introduction to Plant Diversity
From Water to Land
02:45Responses to Heat and Cold Stress
Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.
01:10Sugars as Energy Storage Molecules
Sugar (a simple carbohydrate) metabolism (chemical reactions) is a classic example of the many cellular processes that use and produce energy. Living things consume sugar as a major energy source because sugar molecules have considerable energy stored within their bonds. Consumed carbohydrates have their origins in photosynthesizing organisms like plants. During photosynthesis, plants use the energy of sunlight to convert carbon dioxide gas into sugar molecules, like glucose. Because this...
01:05Proteoglycans
Glycans, a class of complex heterogeneous molecules, can be covalently attached to proteins to form glycosylated proteins that regulate various physiological and pathological processes. Glycosylated proteins or glycoproteins comprise N-linked and O-linked oligosaccharides. O-glycosylation is the most common type of protein glycosylation. Here, glycans attach to the oxygen atom of the hydroxyl groups of Serine or Threonine residues. O-linked glycosylation occurs later in protein processing,...
01:23Glycosaminoglycans
Glycosaminoglycans (GAGs), also known as mucopolysaccharides, are long and linear polymers comprising of specific repeating disaccharides - the amino sugar that can be N-acetylglucosamine or N-acetylgalactosamine, and a uronic acid that is usually glucuronic acid or iduronic acid.
GAGS are found in the extracellular matrix of vertebrates, invertebrates, and bacteria. Due to their polar nature they attract water, and serve as excellent lubricants or shock absorbers in an animal body.
Hyaluronic...
GAGS are found in the extracellular matrix of vertebrates, invertebrates, and bacteria. Due to their polar nature they attract water, and serve as excellent lubricants or shock absorbers in an animal body.
Hyaluronic...