在单细胞水平上循环人类子宫内膜的动态色素重塑
Pavle Vrljicak1, Emma S Lucas2, Maria Tryfonos2
1Warwick Medical School, Division of Biomedical Sciences, University of Warwick, Coventry CV2 2DX, UK; The Zeeman Institute for Systems Biology and Infectious Disease Epidemiology Research (SBIDER), University of Warwick, Coventry CV4 7AL, UK.
Cell reports
|December 7, 2023
概括
这项研究绘制了月经周期期间子宫内膜细胞染色质的变化,揭示了控制胚胎植入的关键基因网络以及可移植元素在子宫受体性中的作用.
科学领域:
- 生殖生物学 生殖生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 基因组学就是基因组学.
背景情况:
- 胚胎植入至关重要的子宫内膜受容性是一个严格规范的过程.
- 激素波动 (雌激素和孕激素) 驱动子宫内膜的周期性变化.
- 控制狭窄的植入窗口的精确分子机制仍然不完全理解.
研究的目的:
- 研究控制人类胚胎植入的基因网络的时间调节.
- 在整个月经周期内映射子宫内膜细胞类型的染色质可访问性和重塑.
- 阐明转录因子和可转移元素在确定子宫内膜受体性的作用.
主要方法:
- 在人类子宫内膜样本上对转化酶可访问的染色质进行测序 (scATAC-seq) 的单细胞检测.
- 染色体免疫沉降测序 (ChIP-seq) 分析.
- 结合染色质可访问性,TF结合和基因表达数据.
主要成果:
- 识别在上皮细胞,层状细胞和免疫细胞中独特的可访问色素区域.
- 在上皮细胞和树皮细胞中划分协调的染色质重塑模式.
- 在决定细胞的监管环境中发现可转移元素 (TE) 选择和TE衍生转录的表达.
结论:
- 这项研究提供了循环人类子宫内膜中染色质动态的细胞分辨率图.
- 通过TF结合和染色体重塑分析,可以获得对受体状态出现的机理洞察.
- 可转移的元素在植入窗口的监管网络中发挥着重要作用.
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