使用二维纳米液体染色学-微自由流电泳分离的循环烯合聚合物微流体设备的表面吸附率的评估
Cecilia C Douma1, Michael T Bowser1
1Department of Chemistry, University of Minnesota, 207 Pleasant Street SE, Minneapolis, Minnesota 55455, United States.
Analytical chemistry
|December 7, 2023
概括
循环烯共聚合物 (COC) 微自由流电泳 (μFFE) 装置显示最小的分析物吸附,性能优于传统的玻璃装置. 这使得COC μFFE对于敏感的,时间解析的微尺度分离非常实用.
科学领域:
- 分析化学 分析化学
- 材料科学 材料科学 材料科学
背景情况:
- 微尺度分离中的表面吸附阻碍了速度,灵敏度和分辨率.
- 循环烯共聚合物 (COC) 是微流体设备的一个有前途的材料,但其在微自由流电泳 (μFFE) 中的表面吸附特性尚未得到充分理解.
研究的目的:
- 为了功能性地描述热印COC μFFE设备的表面吸附.
- 通过评估分析剂峰值扩大,评估COC μFFE适用于时间解析度的测量.
主要方法:
- 使用二维纳米液体色谱 × μFFE (nLC × μFFE) 分离.
- 引入了3到5秒的分析器插头,并测量了时间峰值扩大.
- 测试的小分子染料 (罗达胺123,光素,罗达胺110) 和蛋白质 (肌球蛋白,细胞染色体c).
主要成果:
- 对于小分子染料,观察到最小的吸附,时间峰值宽度为37秒.
- 对标记的肌球蛋白和细胞染色体c观察到适度的吸附,峰值宽度约为20秒.
- 与传统的玻璃设备相比,COC μFFE设备的吸附率明显较低 (峰值宽度以分钟为单位).
结论:
- COC μFFE 设备表现出较低的表面吸附率,使它们适合微尺度分离.
- 对于时间解决的连续检测应用,COC在耐用性,性能和制造方面具有优势.
相关概念视频
Capillary Electrophoresis: Applications
401
Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
401
Supercritical Fluid Chromatography
258
Supercritical fluid chromatography (SFC) provides a beneficial substitute for gas chromatography (GC) and liquid chromatography (LC) for certain samples because it merges the top attributes of both techniques. SFC allows the separation and analysis of compounds that GC or LC does not easily manage. These compounds are traditionally nonvolatile or thermally unstable, making GC unsuitable and lacking functional groups required for HPLC analysis.
SFC utilizes a supercritical fluid mobile phase,...
SFC utilizes a supercritical fluid mobile phase,...
258
Analyte Adsorption and Distribution
669
In certain chromatographic separations, solutes transfer between the mobile phase and the stationary phase via sorption, which typically refers to the process of adsorption. For many chromatographic systems, the sorption process often depends on the polarity of the compounds—an expression of the overall dipole moment within the molecule. During the separation process, there is competition between the solute and solvent for adsorption to the stationary phase. Highly polar compounds and...
669


