通过21核酸相RNAs的mRNA裂变决定了大米中温度敏感的男性不孕
Chuanlin Shi1, Wenli Zou1, Yiwang Zhu1
1Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China.
Plant physiology
|December 7, 2023
概括
在大米中淘汰ARGONAUTE 1d (AGO1d) 会通过影响21核酸相小干扰RNA (phasiRNAs) 来引起对温度敏感的男性不育. 这些phasiRNAs对于男性生育能力和杂交米育种至关重要.
科学领域:
- 植物分子生物学 植物分子生物学
- 遗传学和基因组学 遗传学和基因组学
- 农业科学 农业科学
背景情况:
- 对温度敏感的男性不育性对于双线杂交大米育种至关重要.
- 阿尔戈纳特1d (AGO1d) 淘汰赛以前与这种无菌性有关,影响了分相小干扰RNA (phasiRNA) 途径.
- 参与AGO1d介导不孕症的特定phasiRNAs和目标尚未完全理解.
研究的目的:
- 为了确定特定的phasiRNAs及其目标,负责ago1d米突变体中对温度敏感的男性不育.
- 阐明AGO1d通过哪种机制影响大米的phasiRNA生物发生和功能.
主要方法:
- 多组学分析包括小RNA (sRNA),降解组和转录组测序.
- 在不同温度下对ago1d突变体的基因表达和小RNA配置文件的分析.
- 通过降解组测序识别phasiRNA目标.
主要成果:
- 在低温下,ago1d突变体表现出正常的女性生育能力,但在低温下表现出完全的男性不育性.
- 21-核酸相RNAs是米中最丰富的sRNA物种,对低温敏感,并且在ago1d突变中下调.
- 这些21nt相RNAs对于关键生育和耐寒基因 (例如,EDT1,TDR,OsPCF5,OsTCP21) 的mRNAs的分裂至关重要,它们的损失会导致男性生育缺陷.
结论:
- 21-核酸相RNAs在米中对温度敏感的男性不孕症中发挥着关键作用.
- AGO1d对于这些phasiRNAs的生物发生和功能至关重要,影响男性生育能力.
- 这些发现为双线杂交大米育种计划提供了潜在的应用.
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