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ATOH8的表达受BMP2的调节,在人类子宫内膜干细胞脱离过程中起着关键作用

Rupak Thapa¹, Logan Druessel¹, Liang Ma²

¹Department of Physiology, Southern Illinois University School of Medicine, Carbondale, IL 62901, USA.

|December 7, 2023

概括

原子基本螺旋-环-螺旋转录因子8 (ATOH8) 对于人类子宫内膜脱细胞化至关重要,支持胚胎植入. 骨形态基因蛋白2 (BMP2) 和循环腺单酸盐 (cAMP) 显著调节ATOH8的表达.

科学领域:

生殖生物学生殖生物学
细胞生物学细胞生物学
分子内分泌学分子内分泌学

背景情况:

在分泌阶段和怀孕早期,人类子宫内膜层纤维细胞 (ESFs) 转化为果细胞.
脱二化对于成功的胚胎植入和胎盘发育至关重要.
精确的调节人类ESF决定性的分子机制仍在被阐明.

研究的目的:

为了研究基螺旋环螺旋转录因子8 (ATOH8) 在人类ESF决定化中的作用.
在决定化过程中识别ATOH8表达的上游调节者.

主要方法:

在人体ESF细胞的体外决定化.
使用RNA干扰和等离子体转染来抑制和过度表达ATOH8.
定量实时PCR和西式斑点测试用于评估基因和蛋白质表达.
用BMP2,ALK2/3抑制剂,类固醇和cAMP类似物的治疗.

主要成果:

在体外决定化过程中,ATOH8mRNA和蛋白质水平显著增加,具有核定位.
沉默ATOH8降低了决定性,而过度表达增强了它.
ATOH8调节WNT/FZD-FOXO1通路,影响决定性的标记物.
BMP2和cAMP显著增强了ATOH8的表达,而类固醇没有影响.

结论:

ATOH8在人类社会福利基金的个人化中起着至关重要的作用.
在这个过程中,BMP2和cAMP是ATOH8表达的关键调节者.
在决定化过程中,ATOH8可能起到WNT/FZD-FOXO1通路的上游调节者的作用.

关键词:

个人化的决定性.胚胎植入胚胎的植入这是子宫内膜的内分泌物.这是子宫,子宫.

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