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一个类盐探针用于区分瘤和正常细胞

Jiaying Yu¹, Shen Yuan², Kai Sun³

¹College of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, PR China.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy

|December 7, 2023

概括

一个新的光探针YH-2通过检测细胞内粘度差异来区分瘤细胞. 这种非侵入性探针能够在20分钟内快速进行体内瘤成像,提供了有前途的诊断方法.

科学领域:

生物医学成像技术生物医学成像技术
分子探头分子探头
细胞生物学细胞生物学

背景情况:

开发癌症的非侵入性诊断工具至关重要.
光探头为细胞成像提供高灵敏度和特异性.
细胞内粘度的变化与细胞状态有关,包括恶性瘤.

研究的目的:

开发和描述一种新的光探针YH-2,用于区分癌细胞.
为了研究YH-2在体内瘤成像中的潜力.
评估细胞内粘度与癌细胞检测之间的关系.

主要方法:

合成和YH-2的特征,一个基于黄酸洋盐的光探针.
评估YH-2的光谱特性,包括斯托克斯转移和近红外辐射.
基于细胞内粘度,评估YH-2在癌细胞系 (HeLa,HepG2) 和正常细胞系 (LO2) 之间区分的能力.
在YH-2在尾静脉注射后,在小鼠体内成像研究.

主要成果:

YH-2表现出适当的斯托克斯转移,并在近红外 (NIR) 区域发射.
由于细胞内粘度差异,探针可以有效地区分HeLa,HepG2和LO2细胞.
与正常的LO2细胞相比,瘤细胞显示有机体粘度增加 (内质网膜,线粒体,溶解体).
在注射后20分钟内实现了快速的体内瘤成像,持续成像长达5小时.

结论:

YH-2是一种有效的非侵入性光探针,用于根据细胞内粘度区分癌细胞.
该探针可促进快速和持续的体内瘤成像.
YH-2为体内瘤诊断提供了一个有前途的策略.

关键词:

密度函数理论计算密度函数理论计算区分瘤和正常组织.快速tomur成像成像技术的使用.在NIR-光的光.粘度敏感度对粘度的敏感度

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