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复星通过激活猪中的lncRNAs-KEAP1-NRF2轴来改善肉质特征

Sanbao Zhang¹, Peng Pan², Hongyue Xie²

¹College of Animal Science and Technology, Guangxi University, Nanning, Guangxi 530004, China; Guangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, Guangxi University, Nanning 530004, Guangxi, China.

|December 7, 2023

概括

食复星 (RES) 补充剂通过提高抗氧化能力来提高猪肉质量. RES激活KEAP1-NRF2通路,通过IncRNA调节改善酶活性和肉的特征.

科学领域:

动物科学动物科学
营养生物化学营养生物化学
分子生物学分子生物学

背景情况:

氧化应激对猪肉质量产生负面影响.
复星 (RES) 是一种具有已知的抗氧化特性的多.
了解猪中RES的分子机制对于改善肉类生产至关重要.

研究的目的:

调查食补充白醇对猪抗氧化能力和肉质的影响.
阐明 RES 作用背后的分子机制,重点关注 KEAP1-NRF2 途径.
为了识别参与RES介导抗氧化反应的潜在 lncRNA.

主要方法:

猪骨肌卫星细胞的体外研究.
在体内养试验中,在41天内与24只杂交的野猪进行了试验.
饮食治疗包括基础饮食 (对照) 和RES补充剂 (200,400,600毫克/公斤).
对肉质特征,抗氧化酶活性,KEAP1-NRF2通路激活,分子动力学模拟和RNA测序 (RNA-seq) 的分析.

主要成果:

RES (20μM) 激活了猪细胞中的KEAP1-NRF2通路.
饮食RES (400和600毫克/公斤) 显著改善了肉质和抗氧化酶活性.
分子动力学模拟显示,RES直接结合KEAP1,削弱KEAP1-NRF2的相互作用.
通过RES补充剂调节的RNA-seq确定了KEAP1-NRF2通路相关的lncRNAs.

结论:

饮食中补充白醇可以通过增强抗氧化能力来改善猪肉质量.
该机制涉及激活lncRNA-KEAP1-NRF2轴,增加抗氧化剂水平.
RES通过特定的分子通路作为营养剂,调节抗氧化能力和肉质.

关键词:

抗氧化剂是一种抗氧化剂.这就是KEAP1-NRF2.这是一个LncRNARNA.肉的质量肉的质量猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪复星醇是一种

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相关概念视频

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...