富含甲基酸结构脂的酶性生产过程:开发配方作为一种新的治疗方法
Rodney H Miotti1, Stephanie R do Amaral1, Amanda Noli Freitas1
1Department of Bioprocess Engineering and Biotechnology, São Paulo State University (UNESP), School of Pharmaceutical Sciences, Araraquara, São Paulo, Brazil.
International journal of biological macromolecules
|December 7, 2023
概括
这项研究使用固定酶优化了结构性脂质 (SL) 合成,实现了高整合,并证明了对人类宫腺癌细胞的潜在抗癌活性.
科学领域:
- 生物技术是生物技术.
- 脂质化学 脂质化学
- 生物催化剂是一种生物催化剂.
背景情况:
- 结构性脂质 (SL) 提供量身定制的营养和功能性质.
- 葡萄油和酸是MLM型SL合成的关键基质.
- 酶固定增强生物催化剂的稳定性和可重复使用性.
研究的目的:
- 通过酸分解优化中长中长 (MLM) 结构脂质 (SL) 的合成.
- 评估合成的MLM-SL对癌细胞的细胞毒性作用.
- 研究MLM-SL作为抗癌剂的潜力.
主要方法:
- 使用固定Rhizopus oryzae脂酶,用酸对葡萄油进行酸性溶解.
- 使用中央复合可旋设计 (CCRD) 优化酶负载和反应条件.
- 使用小鼠纤维细胞和人类宫腺癌细胞系评估细胞活力和细胞毒性.
主要成果:
- 优化的生物催化剂在0.3g/g的酶负载下实现了1860 ± 23.4 IU/g的水解活性.
- 酸解反应在24小时内达到大约59%的合并度 (%ID).
- 超过1.75%v/v的MLM-SL度诱导了腺癌细胞的显著细胞死亡 (56-64%),显示出更高的敏感性.
结论:
- 优化的MLM-SL合成是高效的,生物催化剂至少可重复使用五个周期.
- 合成的MLM-SL对人类宫腺癌细胞表现出有前途的细胞毒性作用.
- MLM-SL显示出作为抗癌治疗剂的开发潜力.
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