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Updated: Jul 9, 2025

An Efficient and High Yield Method for Isolation of Mouse Dendritic Cell Subsets

An Efficient and High Yield Method for Isolation of Mouse Dendritic Cell Subsets

Published on: April 18, 2016

在现场的脂质加载激活了以细胞ROS积累为特征的外围树突细胞子集,但损害了它们对原始T细胞的能力

Anette Christ¹, Sanne L Maas², Han Jin³

¹Department of Pathology, Cardiovascular Research Institute Maastricht (CARIM), Maastricht University Medical Centre, Maastricht, Netherlands; Health Office Frankfurt/Main, Frankfurt/Main, Germany.

Free radical biology & medicine

|December 7, 2023

概括

高胆固醇会损害小鼠的树突细胞 (DCs). 脂载体DCs显示活性增加,但刺激T细胞的能力降低,影响动脉样硬化中的免疫反应.

科学领域:

免疫学免疫学免疫学
心血管研究研究心血管研究
代谢障碍代谢障碍代谢障碍

背景情况:

树突细胞 (DCs) 对于适应性免疫和疾病发病至关重要.
高胆固醇血症对DC功能的影响,特别是动脉样硬化,尚不清楚.

研究的目的:

研究高胆固醇血症对小鼠树突细胞子集的直接影响.
评估高脂肪饮食引起的高胆固醇血症对DC区分和功能的影响.

主要方法:

使用低密度脂蛋白受体缺乏 (Ldlr-/-) 的小鼠食高脂肪饮食以诱导高胆固醇血症.
在淋巴细胞组织中分析了树突细胞原始体 (MDP) 频率,增殖和DC子集数.
评估了DC颗粒度,脂质积累,共刺激分子表达,ROS产量和T细胞原始化能力.

主要成果:

高胆固醇血症增加了髓状和树突细胞前体 (MDP) 频率和增殖,但没有改变淋巴状DC亚群数量.
传统的直流体 (cDCs),特别是cDC2,显示颗粒度和脂质积累增加;血细胞直流体受到影响很小.
脂质加载的cDC2表现出增强的共刺激分子表达和ROS积累,但减少了刺激CD4+T细胞的能力.

结论:

在高胆固醇血症中,外围cDC2子集积累脂质,变得过度活化,ROS增加,但T细胞原始化受损.
这些发现对了解如动脉样硬化和高脂血症等代谢障碍中的免疫反应有意义.

关键词:

树突性细胞树突性细胞超高胆固醇血症是什么情况在ROS的积累过程中,ROS的积累在T细胞原始化过程中.

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