从地表水中分离出来的扩展光谱β-lactamase产生Citrobacter物种的基因组景观
Lee-Hendra Chenhaka1, Deidré A B Van Wyk2, Charlotte Mienie1
1Unit for Environment Science and Management, Microbiology, North-West University, Potchefstroom campus, Private Bag X6001, Potchefstroom, 2520, South Africa.
BMC genomics
|December 7, 2023
概括
这项研究在南非地表水中确定了产生扩展谱β-乳酸酶 (ESBL) 的Citrobacter物种. 这些多抗药性病原体是健康问题之一,因为它们可能在水生环境中传播.
科学领域:
- 环境微生物学环境微生物学
- 基因组学就是基因组学.
- 抗微生物耐药性 抗微生物耐药性
背景情况:
- 杆菌物种是格拉姆阴性机会性病原体,经常与医院感染有关.
- 这项研究描述了从南非西北省的地表水中分离出来的四种Citrobacter物种.
研究的目的:
- 来自地表水中的Citrobacter物种的特征,重点关注抗微生物耐药性和毒性基因.
- 确定这些环境孤立的基因组和基因组景观.
主要方法:
- 现型抗微生物敏感性测试以检测扩展光谱β-乳酸酶 (ESBL) 生产.
- 全基因组测序用于分析耐药性,毒性基因和移动遗传元素.
- 在的分类学识别使用多位置序列类型和平均核酸标识,以及泛基因组分析.
主要成果:
- 鉴定了C. braakii,C. murliniae和C. portucalensis菌株,其中 blaCMY-101和 blaCMY-59 ESBL基因的流行率显著.
- 在C. portucalensis中检测到了宏化物和氨基糖化物排泄基因,以及醇抗性基因qnrB13.
- 确定了与囊合成和 siderophore 系统相关的等离子体复制体类型和基因,表明潜在的致病性.
结论:
- 这些产生ESBL的Citrobacter物种的基因组和新兴特征令人担忧,因为它们可能在淡水系统中传播.
- 这些多药耐药 (MDR) 病原体在水生环境中的存在凸显了影响人类,动物和环境健康的一种健康问题.
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