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破碎-1/Notch1信号通路促进了组织工程心脏门的构建
Kang Liu1, Zhang-Yan Wei2, Xue-Hong Zhong3
1Department of Thoracic surgery, Fuyang Sixth People's Hospital, Fuyang, China.
Tissue engineering. Part A
|December 8, 2023
概括
激活Jagged-1/Notch1通路可以增强组织工程心脏膜的内皮细胞功能. 这促进了细胞的增殖,迁移和粘附,这对于门的修复和发育至关重要.
科学领域:
- 生物材料科学 生物材料科学
- 再生医学是一种再生医学.
- 心血管生物学 心血管生物学
背景情况:
- 组织工程心脏门 (TEHVs) 为治疗心脏膜疾病提供了一个有希望的替代方案.
- 诺奇信号通路对于先天性心脏膜发育至关重要.
研究的目的:
- 探索Notch信号通路在TEHV构造中的作用.
- 评估Notch通路激活对TEHVs内内皮质细胞行为的影响.
主要方法:
- 来自脂肪的干细胞分化成诱导的内皮细胞.
- 细胞接受了Jagged-1 (JAG-1) 或g-分泌酶抑制剂 (DAPT) 的治疗,以调节Notch信号.
- 分析了细胞表型,表皮-介质细胞过渡 (EMT) 标记物和粘附蛋白.
- 脱细胞化的猪大动脉支架被用经过处理的细胞重新细胞化.
主要成果:
- 与DAPT不同的是,JAG-1治疗增加了细胞增殖和迁移,同时减少了细胞亡.
- 口激活调高了EMT标记物 (E-cadherin,N-cadherin) 和粘附蛋白 (paxillin,fibronectin) 的调节.
- 激活的Notch信号导致了门表面更密集的细胞排列.
结论:
- 激活JAG-1/Notch1通路可以增强内皮细胞的增殖,迁移和粘附.
- 这些效应有利于矩阵结合,并加速TEHV内皮化.
- 诺奇通路是开发功能性TEHV的关键调节器.
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