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在vivo血管化室用于胚胎脏的植入

Peter Viktor Hauser^1,2, Lifu Zhao¹, Hsiao-Min Chang^1,2

¹Division of Research, Renal Regeneration Laboratory, VAGLAHS at Sepulveda, North Hills, California, USA.

Tissue engineering. Part C, Methods

|December 8, 2023

概括

研究人员开发了一种新的可植入室,用于在小动物中快速进行组织血管化. 这种设备成功地支持胚胎脏生长,克服了组织工程中的一个主要障碍.

科学领域:

生物医学工程生物医学工程
再生医学是一种再生医学.
血管生物学血管生物学

背景情况:

血管化对于工程组织的生存和功能至关重要.
目前的前血管化方法是有限的,通常需要微手术,并限制大动物的使用.
缺血性损伤和缺氧是组织植入的重大挑战.

研究的目的:

开发一种可植入的装置,用于小动物的血管前置.
评估该设备在胚胎脏血管化的有效性.
评估不同生物材料对血管化速度的影响.

主要方法:

一个可植入的腔室被设计并放置在腹腔大动脉和下静脉之间.
室内装满了 I 型原蛋白,Matrigel 或 Matrigel 与血管内皮生长因子 (VEGF).
鼠标胚胎脏 (E12.5) 被植入预血管化和非预血管化腔室进行比较.

主要成果:

在48小时内检测到内皮细胞和血管网络.
血管化速度随着室内的负荷而有显著变化:7天 (原I),4天 (马特里格尔) 和2天 (马特里格尔 + VEGF).
在前血管化腔室中的胚胎脏显示出显著的生长和发育,与非前血管化腔室中的胚胎脏不同.

结论:

开发的前血管化室是植入组织和小型动物模型中的工程结构血管化的有效工具.
这项技术促进了快速的血管化,可能减少缺血性损伤.
需要进一步优化以控制血管内皮细胞的方向性和血管化密度.

关键词:

在 AV 循环 AV 循环中.开发工程是发展工程.通过室内流动的流量.再生医学是一种再生医学.组织工程是组织工程.血管化室的血管化室

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