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Zhou Xu^1,2, Andreas Arkudas¹, Muhammad Azeem Munawar³

¹Laboratory for Tissue Engineering and Regenerative Medicine, Department of Plastic and Hand Surgery, University Hospital of Erlangen, Friedrich-Alexander-University Erlangen-Nürnberg (FAU), Erlangen, Germany.

Tissue engineering. Part A

|December 8, 2023

概括

施万细胞 (SC) 在在纳米纤维上培养的工程骨肌肉中没有显著改善肌体发生或内化. 在这种组织工程模型中,植入的神经分支的存在似乎对功能发展更为关键.

科学领域:

生物材料科学生物材料科学
组织工程是组织工程.
再生医学是一种再生医学.

背景情况:

内化和血管化对于功能性骨肌肉组织工程至关重要.
对齐的纳米纤维作为有益的生物相容矩阵,用于在3D结构中进行细胞播种.

研究的目的:

为了研究施万细胞 (SC) 对肌细胞 (Mb) 和基性介质体细胞 (ADSC) 在聚-ɛ-caprolactone (PCL) -原蛋白I-纳米纤维支架中的共同培养的影响.
在大鼠动脉静脉循环模型中评估血管化,免疫反应,肌原分化和内置.

主要方法:

人类Mb/ADSC和Mb/ADSC/SC共同种植被播种在PCL-原I-纳米纤维支架上.
在EPI循环模型中,在免疫缺陷大鼠的EPI循环模型中植入了4周的支架.
进行了组织学染色和基因表达分析.

主要成果:

两组Mb/ADSC和Mb/ADSC/SC都表现出类似的血管化和免疫活动.
在两组中都观察到肌源性差异化,没有显著差异.
在这两种系统中都存在神经肌肉结节形成的证据.

结论:

在这种骨肌肉组织工程模型中,添加SC并没有显著增强肌体发生或内置.
植入的运动神经分支可能在功能发展中发挥了比SC存在更重要的作用.

关键词:

这是一个EPI循环.电纳米纤维的纳米纤维.介质细胞层细胞.骨髓质细胞的细胞质.肌源性差异化的分化施万的细胞是斯万的细胞.

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