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微RNA-146b对牛胚胎的发育和质量产生负面影响

Krishna Chaitanya Pavani¹, Guan XueFeng², Jayendra Chunduru³

¹K Pavani, Department of Internal Medicine, Reproduction and Population Medicine, University of Ghent, Gent, Belgium.

Reproduction (Cambridge, England)

|December 8, 2023

概括

携带在细胞外囊泡 (EVs) 中的微RNA (miRNAs) 影响胚胎发育. 在非母细胞囊胚胎和EV中过度表达的miR-146b降低了胚胎质量并增加了细胞亡,揭示了关键的调节机制.

科学领域:

生殖生物学生殖生物学
分子生物学分子生物学
发育生物学是发展生物学.

背景情况:

细胞外囊泡 (EV) 中的微RNA (miRNA) 对胚胎发育至关重要.
控制胚胎细胞发育和质量的分子机制在很大程度上仍未被定义.
牛胚胎分泌的细胞外囊泡 (EV) 含有差异表达的miRNAs.

研究的目的:

研究miR-146b对牛胚胎发育和质量的作用.
确定miR-146b对胚胎细胞形成和亡的功能影响.
在胚胎早期发育过程中识别由miR-146b调节的基因.

主要方法:

用RT-qPCR量化胚胎和EV中的miR-146b表达.
用miR-146b模仿剂和抑制剂补充胚胎培养基.
对经过治疗的胚胎进行转录组分析,以评估基因表达变化.

主要成果:

在非胚囊胚胎及其分泌的EV中,miR-146b被上调调节.
miR-146b模仿胚胎质量降低和囊胚率降低,而抑制剂提高了质量.
miR-146b调节了参与细胞亡,细胞分化和转录的基因.

结论:

在质量差的牛胚胎及其 EVs 中,miR-146b 过度表达.
miR-146b 负面调节胚胎发育并促进细胞亡.
miR-146b是改善胚胎质量的潜在生物标志物和治疗点.

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相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...