氧化成形的功能性基因和细菌参与了脱和分散性酸盐的降解,从不同的土中减少
Dandan Pan1,2,3, Pengcheng Chen4, Guang Yang1,3
1SCNU Environmental Research Institute, Guangdong Provincial Key Laboratory of Chemical Pollution and Environmental Safety & MOE Key Laboratory of Theoretical Chemistry of Environment, South China Normal University, Guangzhou 510006, China.
Environmental science & technology
|December 8, 2023
概括
由于微生物酸盐的减少,在无氧环境中铁的氧化会改变微生物群落和基因表达. (Fe) 添加促进了脱化,而不是非仿真性酸盐降解为 (DNRA),影响了循环.
科学领域:
- 环境微生物学环境微生物学
- 生物地质化学生物地质化学
- 气循环是指气循环的过程.
背景情况:
- 微生物酸盐降解与Fe (II) 氧化相结合,影响了氧化排放和无毒环境中的可用性.
- 铁二氧化会导致细胞化,潜在地抑制细菌活动.
- 铁二氧化和细胞对功能基因和细菌的影响,这些基因和细菌参与了脱和异样化酸盐降解为 (DNRA) 的过程,目前尚不清楚.
研究的目的:
- 研究Fe (II) 氧化和随后的细胞层如何影响微生物群落和参与脱和DNRA的功能基因.
- 了解特定细菌属和基因在酸盐降低条件下对Fe (II) 添加的反应.
主要方法:
- 在降低酸盐的条件下,从米土壤中丰富细菌,有或没有Fe (II) 添加.
- 对酸盐减少,酸盐积累,N2O和NH4+生产的监测.
- 使用16S rRNA基因测序和功能基因量化 (例如,napAB,nirS,norBC,nosZ,nirBD,narG,nrfA) 来分析微生物社区组成.
主要成果:
- 由于快速的Fe (II) 氧化和细胞化,Fe (II) 添加减缓了酸盐的减少和增加了酸盐的积累.
- 与单独酸盐相比,Fe (II) 和酸盐的氧化积较低,总体N2O和NH4+产量较低.
- 二氧化的添加导致了土壤中类似的微生物群落结构,其中主导的是德克罗罗莫纳斯,阿佐斯皮拉和伪莫纳斯,并改变了关键脱和DNRA基因的丰富性,有利于周等离子体脱和细胞质酸盐减少为.
结论:
- 铁二氧化和细胞层显著塑造了与循环相关的微生物群落和基因表达.
- (Fe) 添加促进了DNRA上的脱化途径,Dechloromonas在酸盐到N2O的减少中发挥了关键作用,Thauera参与了周等离子体酸盐减少和细胞质DNRA.
- 这些发现为在无氧环境中Fe (II) 氧化和细胞化反应中脱和DNRA细菌的生态作用提供了新的见解.
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