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直接使用4-APEBA衍生物的亚格基微生物相互作用的天然碳产品的质谱成像

Dušan Veličković¹, Kevin J Zemaitis¹, Arunima Bhattacharjee¹

¹Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington, USA.

|December 8, 2023

概括

这项研究引入了一种用于质谱成像的新型化学衍生方法,能够详细可视化微生物共同培养中的300多种代谢物. 这一突破增强了对生物膜中的微生物相互作用和代谢过程的理解.

科学领域:

微生物生态学微生物生态学
分析化学分析化学
代谢学代谢学代谢学

背景情况:

亚利法性碳素酸,和对于微生物适应至关重要,但它们在生物膜中的空间分布尚不清楚.
现有的分子成像策略不足以解决这些生物活性分子在复杂的微生物群落.

研究的目的:

开发和应用一种与 MALDI-MSI 结合的新型组织化学衍生 (OTCD) 方法,用于空间代谢学.
调查形碳基代谢物在细菌-真菌共同培养中的空间分布和潜在作用.

主要方法:

组织上化学衍生物化 (OTCD) 使用4 - - - - - - - - - - - - - - - - - - - - - 甲 (二甲基) 乙氧化 (乙氧化) 胺二化物 (4-APEBA).
在Bacillus subtilis和Fusarium sp.上进行矩阵辅助激光脱/电离质谱成像 (MALDI-MSI). 共同的文化.共同的文化.
分析了300多种含有碳酸的非性代谢产物.

主要成果:

在空间上解析了300多种代谢物,包括以前无法检测到的子,化物和碳素酸.
观察到不同的空间模式,表明在微生物相互作用期间细胞间代谢物交换和差异调节.
在没有正交分离的情况下成功区分异构和异构代谢物.

结论:

基于4-APEBA的OTCD MALDI-MSI是一种强大的工具,用于在现场研究跨王国微生物相互作用.
这种方法显著扩大了代谢覆盖范围,并提高了微生物共同培养中的代谢物识别信心.
这些发现为在微生物群落和生物工程应用中对酸碳基的代谢作用提供了新的见解.

关键词:

这种细菌是 Bacillus subtilis.这里是 Fusariumarium .马尔迪马尔迪马尔迪马尔迪阿尔德化物是什么意思碳素酸是碳素酸中的一种.子子子子代谢生物组的代谢生物组

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