在结肠直肠癌中,mRNA拾取物分解酶的新兴作用和临床含义
Jan Dimberg1, Levar Shamoun2, Gustaf Johansson3
1Department of Natural Science and Biomedicine, School of Health and Welfare, Jönköping University, Jönköping, Sweden.
Pathology, research and practice
|December 8, 2023
概括
垃圾分类酶 (DcpS) 在结肠直肠癌 (CRC) 中升高,并与较差的生存率有关. 沉默DcpS增加了癌细胞的增殖,这表明DcpS是CRC的潜在预后因素.
科学领域:
- 分子生物学分子生物学
- 在瘤学瘤学.
- 基因表达规范 基因表达规范
背景情况:
- RNA周转是基因表达的关键调节者.
- 清除者分解酶 (DcpS) 在RNA循环中发挥作用.
- 在结直肠癌 (CRC) 中了解DcpS对于开发新的治疗策略至关重要.
研究的目的:
- 为了研究dcpS在CRC组织中的表达.
- 评估DcpS在CRC患者的预后意义.
- 为了识别DcpS可能准的基因.
主要方法:
- 免疫组织化学用于DCPS定位.
- 西方斑块和qPCR用于蛋白质和mRNA表达分析.
- 通过siRNA介导的基因沉默和基因阵列分析来研究DcpS功能.
- 基氧氨 (BRDU) 纳入试验用于扩散研究.
主要成果:
- 在正常和CRC组织的上皮细胞中观察到DCPS的表达.
- 在CRC患者中,DcpS mRNA和蛋白质水平显著上调.
- 较低的DcpSmRNA水平与癌症特异性存活率降低相关 (HR=4.7),特别是在直肠癌和扩散癌症中.
- 在Caco-2细胞中抑制DcpS导致了参与RNA处理和细胞周期的基因的改变表达,并增加了23%的增殖.
结论:
- DcpS在结肠直肠癌中受到上调,并与患者存活率较差有关.
- DcpS可以作为CRC的潜在预后生物标志物.
- 需要在更大的队列中进一步验证,以确认DcpS的临床意义.
相关概念视频
Nuclear Export of mRNA
7.7K
Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
7.7K
Nonsense-mediated mRNA Decay
10.6K
The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
10.6K
mRNA Stability and Gene Expression
5.6K
The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
Cis-acting Elements involved in mRNA stability
5.6K
MicroRNAs
3.0K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K
RNA Editing
9.0K
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
9.0K
Pre-mRNA Processing: Modification of pre-mRNA Ends
9.3K
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps...
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps...
9.3K


