相关实验视频

Updated: Jul 9, 2025

An Optimized Protocol for Electrophoretic Mobility Shift Assay Using Infrared Fluorescent Dye-labeled Oligonucleotides

An Optimized Protocol for Electrophoretic Mobility Shift Assay Using Infrared Fluorescent Dye-labeled Oligonucleotides

Published on: November 29, 2016

具有大斯托克斯转移的近红外光探测器用于特定检测氨酸

Jirui Yu¹, Ji Fan¹, Yanxi Song²

¹College of Chemistry and Chemical Engineering, Donghua University, Shanghai 201620, China.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy

|December 8, 2023

概括

开发了一种新的近红外光探测器CL,用于检测素 (Lys). 这种探测器表现出明显的颜色变化和专门用于Lys的增强光,使其能够进行敏感和稳定的检测.

科学领域:

分析化学分析化学
有机化学有机化学
生物化学生物化学

背景情况:

氨基酸检测在生物和医学诊断中至关重要.
现有的传感方法往往缺乏特异性或灵敏度.
对特定氨基酸的选择性探针的开发是一个活跃的研究领域.

研究的目的:

为了合成和描述一种新的近红外 (NIR) 光探测器,CL.
评估探针对氨基酸和生物醇的选择性和敏感性.
探索探测器在实际传感应用中的潜力.

主要方法:

基于库马林 - 迪西亚诺伊索福的光探针CL的合成.
对探头对各种分析物的反应进行光谱分析 (光和色度分析).
检测极限的确定,静脉测量和pH稳定性的确定.
使用1H NMR光谱测量阐明机制.

主要成果:

探测器CL显示了对氨酸 (Lys) 的高选择性,而不是其他氨基酸和生物醇.
在添加Lys后,在702nm时观察到显著的光增强和从黄色变为粉红色的颜色变化.
探测器表现出0.51μM的低检测极限和1:1的静电测量与Lys.
探针CL在广泛的pH范围 (4.0-10.0) 中显示出出色的稳定性.

结论:

开发的CL探头是一个双模态 (光和色度) 传感器,具有很大的斯托克斯转移.
它为氨酸提供了高度特定和实用的传感能力.
探测器的稳定性和灵敏性使其适合潜在的生物和环境监测.

关键词:

颜值测量颜色测量方法颜色测量方法光探测器探测器可以发光.氨酸 (lysine) 是一种氨酸.接近红外的近红外.斯托克斯的轮班正在改变.

更多相关视频

Real-time Monitoring of Ligand-receptor Interactions with Fluorescence Resonance Energy Transfer

Real-time Monitoring of Ligand-receptor Interactions with Fluorescence Resonance Energy Transfer

Published on: August 20, 2012

Imaging Amyloid Tissues Stained with Luminescent Conjugated Oligothiophenes by Hyperspectral Confocal Microscopy and Fluorescence Lifetime Imaging

Imaging Amyloid Tissues Stained with Luminescent Conjugated Oligothiophenes by Hyperspectral Confocal Microscopy and Fluorescence Lifetime Imaging

Published on: October 20, 2017

相关概念视频

Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...