异quercitrin通过Nrf2/ROS/NF-κB通路减弱了类风湿性关节炎中骨质细胞介导的骨质损失
Yan Liu1, Tian-Qi Li2, Jin Bai2
1Lanzhou University, Lanzhou 730000, China; Tianjin Institute of Environmental and Operational Medicine, Tianjin 300050, China.
Biochimica et biophysica acta. Molecular basis of disease
|December 8, 2023
概括
一种天然化合物异基素通过激活Nrf2 (核因子红色素-2相关因子2) 来抑制类风湿性关节炎 (RA). 这阻断了RANKL诱导的信号,减少了骨质结晶形成和RA中的骨损失.
科学领域:
- 生物化学 生物化学
- 免疫学 免疫学 免疫学
- 药理学 药理学是指药理学的学科.
背景情况:
- 类风湿性关节炎 (RA) 涉及过度的骨质细胞形成.
- 由RANKL诱导的ROS-to-NF-κB通路调节骨质细胞形成.
- 关于 Nrf2 在 RANKL 诱导的 NF-κB 激活中的作用进行了讨论.
研究的目的:
- 通过Nrf2. 调查异基是否通过Nrf2. 调节RANKL诱导的NF-κB激活.
- 为了确定异quercitrin对骨质细胞形成和RA相关的骨损失的影响.
主要方法:
- 在体外骨质细胞分化试验 (TRAP染色,F-actin,再吸收坑).
- ROS检测 (Mitosox),基因/蛋白质表达分析 (qRT-PCR,西部斑,免疫光).
- 在体内RA建模和骨损失评估.
主要成果:
- 异quercitrin 抑制了骨质细胞生成,骨解功能和ROS生成.
- 异quercitrin激活了Nrf2并抑制了NF-κB的表达.
- Nrf2激活对于异quercitrin对NF-κB的抑制作用至关重要.
- 在RA模型中,异quercitrin治疗减弱了骨质损失.
结论:
- 由异基激活的Nrf2抑制了RANKL诱导的ROS-to-NF-κB通路.
- 这种机制有效地抑制了骨质结晶发生和RA的骨损失.
- 异quercitrin显示出作为RA治疗剂的潜力.
相关概念视频
NF-κB-dependent Signaling Pathway
7.4K
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
NF-κB-dependent Signaling Mechanism
The...
7.4K
Osteoclasts in Bone Remodeling
3.0K
Osteoclasts are cells responsible for bone resorption and remodeling. They originate from hematopoietic progenitor cells present in the bone marrow. Numerous progenitor cells fuse to form multinucleated cells, each with 10-20 nuclei. A single osteoclast has a diameter of 150 to 200 µM. These cells have ruffled borders that break down the underlying bone tissue and release minerals such as calcium into the blood in bone resorption. Osteoclasts cling to bones with their ruffled edges during...
3.0K
The JAK-STAT Signaling Pathway
8.9K
Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as SH2...
8.9K
Bone Disorders
3.5K
Aging and its effect on bone remodeling is the most common cause of bone disorders. In young and healthy people, bone deposition and resorption happen at an equal rate to maintain optimal bone health.
Bone deposition is also affected by the levels of sex hormones like estrogen and testosterone that promote osteoblast activity and bone matrix synthesis. When the level of these hormones decreases due to aging, it causes a reduction in bone deposition. As a result, bone resorption by osteoclasts...
Bone deposition is also affected by the levels of sex hormones like estrogen and testosterone that promote osteoblast activity and bone matrix synthesis. When the level of these hormones decreases due to aging, it causes a reduction in bone deposition. As a result, bone resorption by osteoclasts...
3.5K
Hormones and Bone Tissue
2.7K
The endocrine system produces and secretes hormones, which interact with the skeletal system. These hormones control bone growth, maintain bone once it is formed, and remodel it.
Hormones That Influence Osteoblasts and/or Maintain the Matrix
Several hormones are necessary for controlling bone growth and maintaining the bone matrix. The pituitary gland secretes growth hormone (GH), which, as its name implies, controls bone growth. This happens in several ways: first, it triggers chondrocyte...
Hormones That Influence Osteoblasts and/or Maintain the Matrix
Several hormones are necessary for controlling bone growth and maintaining the bone matrix. The pituitary gland secretes growth hormone (GH), which, as its name implies, controls bone growth. This happens in several ways: first, it triggers chondrocyte...
2.7K


