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Updated: Jul 9, 2025

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Assays for the Degradation of Misfolded Proteins in Cells
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切割压力作为蛋白质基础疗法降解的驱动因素:比罪祸首更多的同谋
Camilla Moino1, Fiora Artusio1, Roberto Pisano1
1Department of Applied Science and Technology, Politecnico di Torino, 24 Corso Duca degli Abruzzi, Torino 10129, Italy.
International journal of pharmaceutics
|December 8, 2023
概括
由于剪切应力的蛋白质降解会影响治疗疗效和患者安全. 本综述详细介绍了在制药加工过程中切割应力对蛋白质稳定性的影响,并提供了缓解策略.
科学领域:
- 生物化学 生物化学
- 制药科学 制药科学
- 化学工程是化学工程的重要组成部分.
背景情况:
- 蛋白质降解是基于蛋白质的疗法的一个关键问题,可能会改变生物活性并引起患者不良影响.
- 切割应力是蛋白质降解的重要因素,自20世纪70年代以来已被广泛研究.
- 制药加工涉及混合,送和过等单元操作,使药物暴露于剪切和界面应力.
研究的目的:
- 审查与蛋白质结构相关的剪切应力的基本原理.
- 提供生物制药配方中蛋白质降解驱动因素的概述.
- 讨论剪切应力对不同单位运营中的蛋白质稳定性的影响,并建议减缓策略.
主要方法:
- 文献综述,重点关注剪切应力机制和蛋白质降解.
- 在制药生产中遇到的不同剪切应力条件下的蛋白质稳定性的分析.
- 检查用于研究切割应力对蛋白质的影响的设备.
主要成果:
- 剪切应力,与界面应力一起,可以在制药加工过程中显著促进蛋白质降解.
- 不同的单元操作造成不同程度的剪切应力,影响蛋白质的稳定性.
- 了解这些影响对于保持生物制药产品的质量和安全至关重要.
结论:
- 在制药加工过程中最大限度地减少剪切应力暴露对于保持蛋白质完整性至关重要.
- 实施适当的策略和使用专门的设备可以帮助减轻剪切诱导的蛋白质降解.
- 对剪切应力效应和控制方法的进一步研究将有助于开发稳定的基于蛋白质的治疗方法.
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