用Illumina MethylationEPIC BeadChip测定分析的人类DNA甲基化的纵向变化和变化及其对法医年龄预测的影响
Mie Rath Refn1, Mikkel Meyer Andersen2,3, Marie-Louise Kampmann2
1Section of Forensic Genetics, Department of Forensic Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, 2100, Copenhagen, Denmark. mrefn@sund.ku.dk.
Scientific reports
|December 8, 2023
概括
与年龄相关的全表观基因组DNA甲基化变化在15年内对64名个体进行了分析. 虽然大多数与年龄相关的差异甲基化位置 (aDMP) 在人与人之间有所不同,但ELOVL2显示了与年龄相关的甲基化变化.
科学领域:
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 基因组学就是基因组学.
- 衰老研究研究 衰老研究
背景情况:
- 基因甲基化是调节基因表达的关键表观遗传机制.
- 表观遗传修饰,包括DNA甲基化,随着年龄的增长而动态变化.
- 了解与年龄相关的甲基化模式对于生物和法医应用至关重要.
研究的目的:
- 在64个个体的队列中,在15年的时间内调查整个表观基因组的DNA甲基化概况.
- 为了确定与年龄相关的差异甲基化CpG位置 (aDMPs) 并评估它们的可变性.
- 评估之前识别的法医年龄预测标记的实用性.
主要方法:
- 使用了Illumina EPIC850k阵列,用于表观基因组范围内的甲基化分析.
- 采用混合效应模型分析纵向DNA甲基化数据.
- 对所有已识别的aDMP和法医标记进行了年龄相关变化和个体间变异的比较.
主要成果:
- 确定了2821个与年龄相关的差异甲基化CpG位置 (aDMPs),年平均变化为0.18%.
- 观察到大多数aDMPs的基线甲基化和变化方向的显著个体间变化.
- 法医年龄预测标记显示了更大的甲基化变化,但仍然表现出个体间的变化.
结论:
- DNA甲基化模式随着年龄的增长而变化,但具有实质性的个体间变异性.
- 在ELOVL2中的CpG位点cg16867657显示了与年龄相关的均甲基化变化,突出了其作为可靠年龄预测者的潜力.
- 虽然法医标记具有信息性,但它们的变化性需要进一步细化,以便精确地估计年龄.
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