相关实验视频

Updated: Jun 18, 2026

Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform

Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform

Published on: January 30, 2017

脂毒性对线粒体功能和表观遗传编程在牛体内胚胎生产过程中的影响

Ben Meulders¹, Waleed F A Marei^2,3, Inne Xhonneux²

¹Laboratory of Veterinary Physiology and Biochemistry, Department of Veterinary Sciences, Gamete Research Centre, University of Antwerp, Wilrijk, Belgium. Ben.Meulders@uantwerp.be.

Scientific reports

|December 8, 2023

概括

孕产妇的代谢障碍可能会损害卵细胞的发育,影响胚胎表观遗传和线粒体功能. 这项研究揭示了棕酸暴露如何破坏牛胚胎DNA甲基化和基因素修饰,影响ATP生产和基因表达.

科学领域:

生殖生物学生殖生物学
发展生物学发展生物学
线粒体生物学线粒体生物学

背景情况:

孕产妇的代谢障碍对卵细胞发育构成风险.
脂毒性可能会破坏早期胚胎表观遗传编程.
代谢压力,表观遗传学和卵细胞中的线粒体功能之间的联系尚不清楚.

研究的目的:

研究病理生理学棕酸 (PA) 度对牛胚胎表观遗传模式的影响.
为了确定这些表观遗传变化是否与线粒体功能障碍和ATP产生有关.
在PA暴露下检查表观遗传调节基因表达的变化.

主要方法:

使用牛体外模型进行卵细胞成熟和胚胎培养.
评估了DNA甲基化 (5mC) 和基因组修饰 (H3K9ac/H3K9me2) 在胚胎和细胞阶段.
测量了线粒体膜潜力,ATP生产和SOD2基因表达.

主要成果:

聚氨酸暴露增加了生殖细胞中的H3K9ac和H3K9me2,以及Morulae中的5mC和H3K9me2.
观察到在卵细胞和4细胞胚胎中完全抑制糖分 ATP 生产.
在暴露于PA的胚胎中注意到线粒体膜潜力的增加和SOD2表达的减少.

结论:

病理生理学上的棕酸度会在成熟和发育过程中破坏胚胎表观遗传模式.
这些表观遗传变化与显著的线粒体功能障碍和受损的ATP产生有关.
这项研究提供了对母亲代谢压力对早期胚胎发育的影响的新见解.

更多相关视频

In Vitro Culture Strategy for Oocytes from Early Antral Follicle in Cattle

In Vitro Culture Strategy for Oocytes from Early Antral Follicle in Cattle

Published on: July 8, 2020

Use of Bisection to Reduce Mitochondrial DNA in the Bovine Oocyte

Use of Bisection to Reduce Mitochondrial DNA in the Bovine Oocyte

Published on: July 6, 2022

相关概念视频

Animal Mitochondrial Genetics

Animal Mitochondrial Genetics

Among all the organelles in an animal cell, only mitochondria have their own independent genomes. Animal mitochondrial DNA is a double-stranded, closed-circular molecule with around 20,000 base pairs. Mitochondrial DNA is unique in that one of its two strands, the heavy, or H, -strand is guanine rich, whereas the complementary strand is cytosine rich and called the light, or L, -strand. Compared to nuclear DNA, mitochondrial DNA has a very low percentage of non-coding regions and is marked by...

Genomic Imprinting and Inheritance

Genomic Imprinting and Inheritance

Diploid organisms inherit genetic material through chromosomes from both parents. Copies of the same gene are known as alleles. In most cases, both alleles are simultaneously expressed and allow various cellular processes to function optimally. If one of the alleles is missing or mutated, the expression of the other allele can compensate; however, this is not true for all genes.
The expression of some genes depends on which parent passed the gene to the offspring, through a phenomenon known as...