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相关概念视频

Allosteric Proteins-ATCase01:19

Allosteric Proteins-ATCase

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Binding sites linkages can regulate a protein's function.  For example, enzyme activity is often regulated through a feedback mechanism where the end product of the biochemical process serves as an inhibitor.
Aspartate transcarbamoylase (ATCase) is a cytosolic enzyme that catalyzes the condensation of L-aspartate and carbamoyl phosphate to  N-carbamoyl-L-aspartate. This reaction is the first step in pyrimidine biosynthesis. UTP and CTP, the end products of the pyrimidine synthesis...
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Most chemical reactions in cells require enzymes—biological catalysts that speed up the reaction without being consumed or permanently changed. They reduce the activation energy needed to convert the reactants into products. Enzymes are proteins, that usually work by binding to a substrate—a reactant molecule that they act upon.
Enzymes exhibit substrate specificity, meaning that they can only bind to certain substrates. This is mainly determined by the shape and chemical...
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The theory of catalytically perfect enzymes was first proposed by W.J. Albery and J. R. Knowles in 1976. These enzymes catalyze biochemical reactions at high-speed. Their catalytic efficiency values range from 108-109 M-1s-1. These enzymes are also called 'diffusion-controlled' as the only rate-limiting step in the catalysis is that of the substrate diffusion into the active site. Examples include triose phosphate isomerase, fumarase, and superoxide dismutase.
 
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Phosphoinositides and PIPs01:42

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Phosphoinositides are a group of phospholipids containing a glycerol backbone with two fatty acid chains and a phosphate attached to a myoinositol sugar ring. The inositol head group extends into the cytoplasm, where it is modified by adding phosphate groups to form phosphatidylinositol phosphates or PIPs.
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...
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An In Vitro Assay to Detect tRNA-Isopentenyl Transferase Activity
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一种ITPA酶,具有改善的基质选择性.

Nicholas E Burgis1, Kandise VanWormer2, Devin Robbins2

  • 1Department of Chemistry, Biochemistry & Physics, Eastern Washington University, Cheney, WA, 99004, USA. nburgis@ewu.edu.

The protein journal
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PubMed
概括
此摘要是机器生成的。

婴儿ITPA缺陷可能是致命的. 研究人员设计了一种具有增强活性的新型E22D ITPA酶,为开发ITPA相关疾病的新疗法提供了潜在的平台.

关键词:
在DEE 35中,35是DEE.在ITP中,ITP是ITP.在ITPA中,ITPA是ITPA.伊诺西尼 (Inosine) 是一种蛋白质.利巴维林 (ribavirin) 是一种药物.提奥普林是一种类型的氨基酸.

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科学领域:

  • 生物化学 生物化学
  • 遗传学 遗传学 是一个
  • 酶工程是什么? 酶工程是什么?

背景情况:

  • 婴儿对三酸氨酸酸酸酶 (ITPA) 的缺陷可能是致命的,这突显了它在神经发育中的关键但不太了解的作用.
  • ITPA基因多态性影响了利巴维林和氨酸治疗患者的结果,影响了近三分之一的人口.
  • 之前的研究发现了一种E22A ITPA突变,通过局部定向突变发生,增强了ITP水解活性.

研究的目的:

  • 通过理性酶工程来研究ITPA突变体在22位的生物化学特性.
  • 为治疗应用开发一种改进的ITPA酶,具有增强的基质选择性.

主要方法:

  • 在位置22的ITPA基质选择性口袋的合理酶工程.
  • 工程ITPA突变体的生物化学表征,包括基质选择性试验.
  • 对新型ITPA突变物E22D的生物活性进行评估.

主要成果:

  • E22D ITPA突变体对ITP的基质选择性比ATP提高了两倍.
  • E22D ITPA突变体显示,ITP对基质的选择性比GTP提高了四倍.
  • 工程 E22D ITPA 保持了其生物活性,同时展现了增强的 ITP 水解.

结论:

  • 新型E22D ITPA突变体表现出显著增强的基质选择性,并保持生物活性.
  • 像E22D ITPA这样的工程ITPA变体代表了开发新型治疗策略的有希望的平台.
  • 进一步开发E22D ITPA可能会导致ITPA缺陷和相关疾病的治疗方法得到改进.