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在质母细胞多形细胞中,对低氧诱导的长非编码RNA差异表达的分析分析和生物信息学分析

Xiaomin Cai¹, Mengshu Qian², Kui Zhang³

¹Department of Neurosurgery, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, 1665 Kong Jiang Road, Yangpu District, Shanghai, 200092, China.

Biochemical genetics

|December 8, 2023

概括

缺氧会改变长非编码RNA (lncRNA) 在多种质母细胞瘤 (GBM) 细胞中的表达. 确定了六种特定的lncRNAs,其中uc003tnq.3抑制了GBM细胞的增殖和入侵.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
基因组学就是基因组学.

背景情况:

低氧微环境在质母细胞瘤多形 (GBM) 恶性瘤中至关重要.
长非编码RNAs (lncRNAs) 与GBM进展有关,并与缺氧有关,但它们的具体作用尚不清楚.

研究的目的:

在低氧条件下探索GBM细胞中的差异性lncRNA表达特征.
阐明失调的lncRNAs在低氧下GBM发展中的功能性作用.

主要方法:

在低氧U87和T98G细胞中对lncRNA的测序分析.
对差异表达 lncRNAs (DElncRNAs) 的生物信息分析.
定量逆转录聚合酶连锁反应 (qRT-PCR) 验证和功能实验.

主要成果:

在U87和T98G细胞中,分别有1115和597个lncRNAs的调节失调.
改变的lncRNA表达与核酸切割修复,细胞代谢和Hippo信号通路有关.
抑制uc003tnq.3显著抑制了GBM细胞的增殖,入侵和迁移.

结论:

在低氧状态下,lncRNA表达在GBM细胞中显著失调.
包括uc003tnq.3在内的六个已识别的lncRNA在低氧微环境中的GBM发育中起着至关重要的作用.

关键词:

表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式这是一个LncRNARNA.这是一个LncRNA-miRNA-mRNA网络.序列分析是指进行序列分析.

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相关概念视频

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...