高温诱导的m6A表观遗传变化影响猪早期胚胎发育能力
Ming-Hong Sun1, Wen-Jie Jiang1, Xiao-Han Li1
1Department of Animal Science, Chungbuk National University, Chungdae-ro 1, Seowon-gu, Cheongju, Chungbuk, 28644, South Korea.
概括
高温压力通过改变N6-甲基氨酸 (m6A) RNA修饰水平来破坏早期猪胚胎发育. 这种压力会影响关键的发育过程和蛋白质表达,影响胚胎的生存能力.
科学领域:
- 生殖生物学 生殖生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 哺乳动物胚胎发生
背景情况:
- N6-甲基氨酸 (m6A) 是一种影响真核生物基因表达的关键mRNA修饰.
- 在胚胎早期发育过程中,m6A的"写入器"",删除器"和"读取器"的作用至关重要,但尚未完全理解.
- 了解压力下的表观遗传修饰对于繁殖成功至关重要.
研究的目的:
- 为了研究高温 (HT) 应激对早期猪胚胎m6A修饰的影响.
- 确定HT如何影响关键的m6A调节蛋白 (写字器,擦拭器,阅读器) 和整体m6A水平.
- 为了将m6A修饰的变化与热应激猪胚胎的发育结果相关联.
主要方法:
- 猪卵细胞被暴露在高温 (10小时41°C) 中.
- 卵子细胞被伴生遗传激活并培养到胚芽细胞阶段.
- 分析了m6A"写" (METTL3),"擦" (FTO) 和"读" (YTHDF2) 蛋白质的表达水平.
- 量化了m6A修饰水平和热冲击蛋白70 (HSP70) 的表达.
主要成果:
- 高温显著降低了第一极体挤出率和胚胎细胞形成率.
- 暴露于HT会增加m6A"写" (METTL3) 和"读" (YTHDF2) 蛋白的表达.
- 暴露于HT降低了m6A"清除器" (FTO) 蛋白的表达,导致m6A水平升高.
- 在HT条件下,热冲击蛋白70 (HSP70) 表达被上调.
结论:
- 高温压力对猪早期胚胎发育有负面影响.
- 通过影响关键调节蛋白质,HT暴露会改变m6ARNA修饰的平衡.
- 在m6A修饰模式的变化与在热应激下受损的胚胎发育有关.
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