开发和应用潜在的万能微卫星标记物对鱼物种的开发和应用
Daxin Xie1, Nan Yang2, Wencai Xu1
1Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, China.
Animals : an open access journal from MDPI
|December 9, 2023
概括
研究人员开发了49种潜在的通用微卫星标记物,用于羊种类,以评估遗传多样性. 这对于中国危羊种群的保护工作至关重要.
科学领域:
- 基因组学和保护生物学
- 分子生态学分子生态学
背景情况:
- 中国西南地区的种群因息地碎片化和环境变化而面临灭绝危险.
- 评估遗传多样性对于这些物种的进化潜力和保护计划至关重要.
- 需要通用遗传标记来促进羊广泛的遗传多样性评估.
研究的目的:
- 选和开发潜在的普世微卫星标记物,用于鱼物种.
- 为了验证这些标记物在多种属中有效.
- 应用已开发的标记物来评估危羊物种的遗传多样性.
主要方法:
- 471个微卫星位点的全基因组选,这些位点在八种鸟中是共同的.
- 选择和开发119个微卫星标记器.
- 在Phasianidae的14个物种 (11个属) 中进行PCR放大,优化和验证位点.
- 应用49个选定的标记物来评估三种羊的遗传多样性.
主要成果:
- 49个潜在的通用微卫星标记物成功地被确定为鱼物种.
- 这些标记在广泛的属中得到了验证.
- 遗传多样性的评估显示,四川山,血和喉的低水平.
结论:
- 开发的49个微卫星位置对种群遗传学研究具有潜在的普遍性.
- 这些标记物为建立羊遗传研究共享平台提供了重要工具.
- 这些发现凸显了对表现出低遗传多样性的羊种进行有针对性的保护行动的必要性.
相关概念视频
Proteomics
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
iChip
The cultivation of environmental microorganisms has long been hindered by the inability to replicate complex native conditions in vitro. The isolation chip (iChip) addresses this limitation by facilitating the growth of previously uncultivable microorganisms through in situ incubation. Designed for high-throughput microbial cultivation, the iChip comprises hundreds of microchambers, each capable of housing a single microbial cell. These microchambers are loaded with a mixture of molten agar and...


