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在狗中通过初级和二级胆酸调节体内巨细胞反应
Alison C Manchester1, Lyndah Chow1, William Wheat2
1Department of Clinical Sciences, Colorado State University, Fort Collins, CO 80523, USA.
Animals : an open access journal from MDPI
|December 9, 2023
概括
主要和次要胆汁酸 (BAs) 调节犬类巨细胞的免疫反应. 两者都抑制了TNF-α和增强了IL-10,但激活了不同的信号通路,影响了胃肠道炎症.
科学领域:
- 免疫学 免疫学 免疫学
- 胃肠病学 胃肠病学
- 代谢学 代谢学 代谢学
背景情况:
- 胆汁酸 (BAs) 是影响肠道免疫系统的关键代谢物.
- 主要和次要BA具有不同的免疫特性.
- 肠道BA池的组成可能会影响胃肠道炎症状况.
研究的目的:
- 为了比较初级 (胆酸,CA) 和二级BA (石化酸,LCA) 对犬类巨细胞的免疫调节作用.
- 研究CA和LCA对巨细胞因子分泌和TGR5表达的影响.
- 通过使用RNA测序来探索CA和LCA的差异信号通路调制.
主要方法:
- 在BA暴露后评估狗巨细胞因子分泌 (TNF-α,IL-10).
- 胆酸受体TGR5.5的测量表达.
- 利用RNA测序来分析巨细胞对具有和没有CA/LCA的LPS的转录基因反应.
主要成果:
- CA和LCA都抑制了LPS诱导的TNF-α,并增强了巨细胞的IL-10分泌.
- 主要和次要BA都没有改变TGR5表达.
- RNA测序揭示了不同的通路激活:CA调节了II型干扰素和酸受体信号传递,而LCA影响了氧化信号传递和细胞循环调节.
结论:
- 主要和次要胆汁酸积极调节犬类巨细胞的免疫反应.
- 共同影响包括改变的细胞因子配置,而不同的途径激活表明在炎症中的不同作用.
- 这些发现凸显了胆汁酸在狗肠道中复杂的免疫调节作用.
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