WAVE2调节由B细胞抗原受体和整体素诱导的取决于动氨酸的过程
Abhishek Bedi1, Kate Choi1, Connor Keane1
1Department of Microbiology and Immunology, Life Sciences Institute, University of British Columbia, Vancouver, BC V6T1Z3, Canada.
Cells
|December 9, 2023
概括
通过调节actin细胞骨架动态,WAVE2对B细胞扩散和免疫突触形成至关重要. 这项研究揭示了WAVE2.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
- 生物化学 生物化学
背景情况:
- B细胞抗原受体 (BCR) 信号驱动着乙重塑,这对于B细胞功能,如扩散和免疫突触 (IS) 形成至关重要.
- 包括WASp家族成员在内的促动动因子激活Arp2/3复合体,但WAVE2在B细胞中的特定作用仍然未被描述.
研究的目的:
- 研究WAVE2在actin细胞骨架重塑和B细胞功能中的作用.
- 确定WAVE2对B细胞扩散,免疫突触形成和信号传递的贡献.
主要方法:
- 在扩散的B细胞中对WAVE2和Arp2/3复合物的局部化研究.
- 通过RNA干扰 (siRNA) 在B细胞中消耗WAVE2.
- 评估B细胞在固定抗体和纤维菌素上扩散的情况.
- 分析F-actin环形成,actin逆流和整合素介导的粘附.
- 在抗原呈现时评估BCR信号和B细胞激活.
主要成果:
- 在B细胞扩散过程中,WAVE2和Arp2/3在分支的F-actin环上同位.
- WAVE2的枯竭会影响B细胞的扩散,降低F-actin环的厚度,并减少actin逆行流.
- 此外,WAVE2缺乏也阻碍了基于整合素的扩散和actomyosin弧形的形成.
- 降低的WAVE2水平降低了BCR微集群信号传输,IS信号放大,以及抗原驱动的B细胞激活.
结论:
- 在B细胞中,WAVE2是Arp2/3介导的B细胞分支性活性核的关键调节者.
- WAVE2在B细胞扩散,免疫突触动力学和信号放大方面发挥着至关重要的作用.
- 这些发现凸显了WAVE2对淋巴细胞中多种actin依赖过程的重要贡献.
相关概念视频
B Cell Activation and Differentiation
1.7K
The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
1.7K
Intracellular Signaling Affects Focal Adhesions
2.7K
Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
Some...
2.7K
Activation of Integrins
3.5K
Integrins bind ligands and transmit information from outside the cell to inside or vice-versa through an "outside-in signaling" or "inside-out signaling."
In "outside-in signaling," external factors in the extracellular space bind to exposed ligand binding sites on integrins. This causes the inactive protein to undergo a conformational change to become active. Integrins are often clustered on the cell membrane. Repetitive and regularly spaced ligand binding...
In "outside-in signaling," external factors in the extracellular space bind to exposed ligand binding sites on integrins. This causes the inactive protein to undergo a conformational change to become active. Integrins are often clustered on the cell membrane. Repetitive and regularly spaced ligand binding...
3.5K
Mechanism of Lamellipodia Formation
2.6K
Cells migrating in response to external stimuli form lamellipodia, which are thin membrane protrusions supported by a mesh of linked, branched, or unbranched actin filaments. These actin filaments interact with myosin motor proteins, creating the dynamic actomyosin complex within the cytoskeleton. Contractility, or the ability to generate contractile stress, is inherent to the actomyosin complex. It helps cells detect the stiffness of the surrounding ECM and exert contractile force for...
2.6K
T Cell Activation and Clonal Selection
741
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
Naive T cells that have not yet encountered an antigen express two primary CD...
741
Generation of Straight or Branched Actin Filaments
2.9K
The straight or branched structure formation of actin filaments is controlled by nucleating proteins such as the formins and Arp2/3 complex. Formin-mediated assembly results in straight filaments, whereas Arp2/3 protein complex-mediated assembly results in branched actin filaments.
Arp2/3 Complex
Arp2/3 complex is a seven-subunit complex consisting of two proteins similar to actin- Arp2 and Arp3, and five other subunits that help keep Arp2 and Arp3 inactive. When required, the complex is...
Arp2/3 Complex
Arp2/3 complex is a seven-subunit complex consisting of two proteins similar to actin- Arp2 and Arp3, and five other subunits that help keep Arp2 and Arp3 inactive. When required, the complex is...
2.9K


