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两种来自细菌的抗菌和它们的特性

Yujia Zhang¹, Zinuo Meng¹, Shilong Li¹

¹College of Life Sciences, Hebei University, Baoding 071002, China.

Molecules (Basel, Switzerland)

|December 9, 2023

概括

研究人员从Bacillus菌株中分离出抗菌,作为动物料中抗生素的潜在替代品. 这些具有广泛的抗病原体活性和良好的稳定性,为畜牧业减少抗生素提供了有希望的解决方案.

科学领域:

微生物学微生物学
生物化学生物化学
动物科学动物科学

背景情况:

在畜牧业中过度使用抗生素导致抗菌素耐药性,需要替代品.
来自Bacillus物种的抗微生物 (AMP) 对耐药病原体具有广泛的活性.
监管趋势有利于限制或禁止在牲畜料中使用抗生素.

研究的目的:

从Bacillus velezensis和Bacillus inaquosorum菌株中分离和表征抗菌.
评估这些的有效性和稳定性,作为潜在的料抗生素替代品.
确定用于畜牧业开发的新序列.

主要方法:

从料添加剂中分离出百菌大发病9 - 1和百菌无水76 - 1.
使用硫酸沉,离子交换,凝色谱和RP-HPLC净化抗微生物.
通过LC-MS/MS识别分子量和序列.

主要成果:

两种,-I (VFLENVLR, 988.5706 Da) 和-II (FSGSGSGTAFTLR, 1286.6255 Da) 被净化并鉴定出来.
这两种都表现出显著的抗菌活性,对抗黄金葡萄球菌,大麦芽和沙门氏菌.
在热,酸,和蛋白质酶条件下表现出极好的稳定性.

结论:

从Bacillus spp.中分离出来的抗微生物. 它们对关键的畜牧病原体有效.
这些酸具有有利的稳定性,使其适用于动物料应用.
鉴定到的代表着有希望的,安全的替代品,用于传统的抗生素在畜牧业.

关键词:

细菌杆菌是一种这是LC-MS/MS.抗微生物类的抗微生物.最低抑制度 (MIC) 是指最小的抑制度.稳定的稳定性稳定的稳定性

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