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Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography

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Published on: March 12, 2017

在Brightfield显微镜中进行组织图像的断层重建的实验平台

Panteleimon Koudounas¹, Efthymios Koniaris², Ioannis Manolis²

¹Medical Image and Signal Processing Laboratory, Department of Biomedical Engineering, University of West Attica, Egaleo, 12243 Athens, Greece.

Sensors (Basel, Switzerland)

|December 9, 2023

概括

这项研究引入了一种用于生物组织部分的新型断层成像方法,可以实现超越传统显微镜的详细3D可视化. 该技术通过揭示生物样本中隐藏的结构来提高诊断的准确性.

科学领域:

生物医学工程生物医学工程
光学显微镜的使用方法
医疗成像医学成像

背景情况:

对生物组织的微观分析具有挑战性,容易出现错误.
当前的二维成像方法可能会错过厚样本中的关键细节.

研究的目的:

开发生物组织切片的断层成像方法.
为了实现组织结构的详细3D可视化.

主要方法:

开发了一个由ARDUINO控制的定制显微镜阶段.
该阶段允许样品旋转,将光学显微镜转换为CT几何.
基于投影的断层图形重建算法用于光学切片.

主要成果:

实现了令人满意的图像质量.
观察到一些文物,特别是在轴平面图像中.

结论:

在有限的角度进行断层重建允许对任何光学平面进行调查.
这种方法揭示了在传统的二维图像中难以识别的信息.

关键词:

3D组织体积成像 3D组织体积成像癌症癌症癌症癌症癌症组织病理学组织病理学光学显微镜的光学显微镜.

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