已过期的血小板度上升循环:增长因子丰富的生物产品制剂用于FBS替代品
Eun Hye Lee1, So Young Chun2, Bo Hyun Yoon2
1Joint Institute of Regenerative Medicine, Kyungpook National University, Daegu 41566, Republic of Korea.
Journal of clinical medicine
|December 9, 2023
概括
过期的血小板缩物 (PC) 可以被加工成一种富含生长因子的补充剂,用于人类细胞培养. 这种方法优化了冷,解和杀菌,为胎儿牛血清 (FBS) 提供了可行的替代方案.
科学领域:
- 生物技术是生物技术.
- 细胞培养 细胞培养
- 再生医学是一种再生医学.
背景情况:
- 过期血小板缩物 (PC) 是一种含有保存生物活性分子的废物.
- 目前的细胞培养基通常依赖于胎儿牛血清 (FBS),但它有局限性.
- 人类细胞培养需要有效和可持续的补充剂.
研究的目的:
- 开发一种将生长因子从已过期的PC中分离出来的过程.
- 优化条件以最大限度地提高增长因子收益率和稳定性.
- 为了评估衍生生物产品作为细胞培养补充剂的有效性.
主要方法:
- 将至少10个PC单元组合在一起,以最大限度地减少变化.
- 优化冷-解周期 (5个周期:12小时在-80°C冷,5分钟在36°C解).
- 顺序过 (40微米,0.45微米,0.22微米) 用于杀菌和减少损失.
主要成果:
- 增长因子在室温下保持稳定4-6小时,在4°C下24小时,在-80°C下12个月.
- 与FBS相比,生物产品显著增加了人类脏近壁管道上皮细胞的生长率.
- 细胞形态和遗传特征被保留在培养的干细胞和前代细胞中.
结论:
- 一种新的方法有效地从过期的PC中分离出稳定的增长因素.
- 由此产生的生物产品作为人类细胞培养的强效,无FBS补充剂.
- 这种方法为在临床应用中使用过期的血液产品提供了一个可持续的解决方案.
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