血小板激活因子受体表达和人类鼻息肉组织中的lysoplatelet激活因子异型的升调
Jordi Roca-Ferrer1,2, Maria Pérez-González1,2, Isam Alobid1,2,3,4
1Clinical and Experimental Respiratory Immunoallergy (IRCE), Clinic Foundation for Biomedical Research-August Pi Sunyer Biomedical Research Institute (FRCB-IDIBAPS), 08036 Barcelona, Spain.
Journal of clinical medicine
|December 9, 2023
概括
血小板激活因子 (PAF) /受体 (PAFR) 系统在患有鼻息肉 (CRSwNP) 的慢性鼻炎时受到上调,特别是在患有喘的患者中. 在CRSwNP中,Lyso-PAF水平也升高,这表明它在疾病发病过程中发挥了作用.
科学领域:
- 免疫学 免疫学 免疫学
- 鼻科 鼻科是指鼻科的专家.
- 分子生物学分子生物学
背景情况:
- 血小板激活因子 (PAF) /受体 (PAFR) 系统与喘和过敏性鼻炎有关.
- 它在慢性鼻炎 (CRS) 中的具体作用在很大程度上仍未定义.
- 这项研究研究了带有鼻息肉 (CRSwNP) 的CRS中的PAFR和Lyso-PAF,包括喘和NSAID恶化呼吸道疾病 (N-ERD) 等并发症.
研究的目的:
- 为了评估血小板激活因子受体 (PAFR) 表达和Lyso-Platelet-Activating Factor (Lyso-PAF) 异形度在CRS患者的鼻息肉 (NP) 中.
- 将这些水平与健康的鼻腔粘膜 (NM) 进行比较.
- 分析CRSwNP表型之间的差异,包括患有喘和N-ERD的表型.
主要方法:
- 分析来自对照和CRS患者的鼻腔粘膜 (NM) 和鼻息肉 (NP) 组织.
- 通过免疫化学,免疫光,西斑和qPCR评估PAFR蛋白和mRNA.
- 使用质谱测量对Lyso-PAF异型 (C16,C18,C18:1) 的量化.
主要成果:
- 在NM和NP的上皮细胞和下腺体中存在PAFR蛋白.
- 与NM相比,PAFR mRNA表达在所有NP表型中显著更高.
- 从喘患者的NP中,Lyso-PAF异型度较高,与非喘CRSwNP患者相比,C16和C18水平更高.
结论:
- 该PAF/PAFR系统在CRSwNP中表达和活性增加.
- 升高的Lyso-PAF水平,特别是在喘患者中,表明具有显著的病理生理作用.
- 该PAF/PAFR系统是CRSwNP病原体的潜在贡献者.
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