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在缺乏的情况下,在苗的根源中对长非编码RNA的全基因组识别和表征

Dongfeng Qiu^1,2, Yan Wu², Kuaifei Xia³

¹Engineering Research Center of Ecology and Agricultural Use of Wetland, Ministry of Education, Hubei Key Laboratory of Waterlogging Disaster and Agricultural Use of Wetland, College of Life Science, Yangtze University, Jingzhou 434025, China.

Plants (Basel, Switzerland)

|December 9, 2023

概括

长非编码RNAs (lncRNAs) 调节基因表达. 这项研究在低条件下确定了根中的lncRNAs,揭示了它们在使用效率和植物生长中的作用.

科学领域:

植物分子生物学植物分子生物学
基因组学就是基因组学.
生物化学生物化学

背景情况:

长非编码RNAs (lncRNAs) 是真核生物中基因表达的关键调节者.
lncRNAs越来越多地被认为是它们在植物非生物应激反应中的潜在作用,包括使用效率.
了解 lncRNA 功能对于提高作物弹性和产量至关重要.

研究的目的:

识别和描述涉及低 (LN) 条件下大米反应的lncRNAs.
在 LN 压力下研究 lncRNA 和传递 RNA (mRNA) 之间的调控网络.
阐明已识别的 lncRNA 在吸收和植物生长中的特定功能.

主要方法:

在LN处理下对大米根的转录基因测序.
生物信息学分析以确定差异表达的lncRNA和mRNA.
lncRNA-mRNA相互作用网络的构建和分析.
一个特定的LN诱导的lncRNA (lncRNA24320.6) 的分子特征.

主要成果:

在大米根中鉴定了1628个lncRNAs,在LN治疗4h和48h后观察到显著的差异性表达.
构建一个相互作用网络,揭示 lncRNA 和 mRNA 之间的潜在调节关系.
lncRNA24320.6的特征,显示其对黄类3'-氧酶5 (OsF3'H5) 基因的积极调节.
在LN条件下,lncRNA24320.6的过度表达增强了的吸收,并促进了苗的生长.

结论:

lncRNAs在米对饥饿的反应中发挥着重要作用.
lncRNA24320.6是米中使用效率的积极调节者,影响基因表达和植物表型.
这些发现为制定提高使用效率的策略提供了有价值的见解. 在大米和其他作物中.

关键词:

长非编码RNA是什么意思这种气是气.米米饭米饭米饭米饭这就是ssRNA-Seqq.转录组 (transcriptome) 是一个转录组.

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相关概念视频

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...