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石英晶微平衡平台用于SARS-CoV-2免疫诊断
Per H Nilsson1,2, Mahmoud Al-Majdoub3, Ahmed Ibrahim3
1Linnaeus University Centre for Biomaterials Chemistry, Department of Chemistry and Biomedical Sciences, Linnaeus University, SE-39182 Kalmar, Sweden.
International journal of molecular sciences
|December 9, 2023
概括
一种新的石英晶微平衡 (QCM) 免疫测试用于SARS-CoV-2抗体,显示出高可重现性和线性. 这种QCM试验与已建立的电化学发光和化学发光免疫试验有很好的一致性.
科学领域:
- 生物医学工程 生物医学工程
- 免疫学 免疫学 免疫学
- 分析化学 分析化学
背景情况:
- 准确的SARS-CoV-2抗体血清分析对于评估免疫力和跟踪病毒传播至关重要.
- 石英晶体微平衡 (QCM) 是一种无标签的传感器技术,可以实时检测分子结合事件.
- 现有的血清分析,如ECLIA和CLIA,为SARS-CoV-2抗体提供了有价值但独特的检测方法.
研究的目的:
- 开发和评估基于QCM的免疫试验,用于检测SARS-CoV-2特定IgG抗体.
- 评估开发的QCM免疫试验的可复制性和线性.
- 为了比较QCM免疫测试的性能与已建立的商业血清学测试.
主要方法:
- 开发一个QCM传感器平台,实时检测SARS-CoV-2抗体结合.
- 使用日常相关性分析 (r2 = 0.99) 评估试验可重复性.
- 通过在血清和缓冲剂中的连续稀释来评估试验线性 (r2 = 0.96).
- 使用119个人类血清样本,与罗氏ECLIA和YHLO CLIA进行QCM试验的交叉比较.
主要成果:
- QCM免疫试验表现出良好的日常可重复性 (r2 = 0.99) 和线性 (r2 = 0.96).
- 与商业分析的交叉比较显示出良好的连贯性,积极的同意范围为80-100%,负面的同意范围为96-100%.
- 在QCM分析中,在119个样本中发现了48个阳性样本,而罗氏为59个样本,YHLO为52个样本.
结论:
- 开发的基于QCM的SARS-CoV-2 IgG免疫试验具有高度可重现性和线性.
- 该QCM测定表明与已建立的基于ELISA的血清学方法有很好的一致性.
- 抗体标位和结合亲和度等因素可能会影响不同测试平台的结果.
相关概念视频
01:33Enzyme-Linked Immunosorbent Assay
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There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or quantified.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or quantified.
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