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蛋白冠状形成对抗体功能化液体脂质纳米载体的作用

Saúl A Navarro-Marchal^1,2,3, Marina Martín-Contreras⁴, David Castro-Santiago⁴

¹Biopathology and Regenerative Medicine Institute (IBIMER), Centre for Biomedical Research (CIBM), University of Granada, 18100 Granada, Spain.

International journal of molecular sciences

|December 9, 2023

概括

脂质液体纳米囊 (LLNCs) 上的蛋白冠状 (PC) 不会阻止细胞向,但在HER2阳性癌细胞中显著减少40%的细胞吸收.

科学领域:

纳米医学是一种纳米医学.
生物技术是生物技术.
细胞生物学细胞生物学

背景情况:

蛋白质冠状形成是影响纳米粒子-细胞相互作用的关键因素.
有针对性的纳米载体对于有效的药物输送和诊断至关重要.
了解蛋白质吸附如何影响纳米载体功能对于治疗开发至关重要.

研究的目的:

为了研究蛋白冠对多功能纳米粒子细胞相互作用的影响.
为了表征纳米载体的物理化学性质,有和没有蛋白质冠状.
评估冠状蛋白对癌细胞向细胞吸收的影响.

主要方法:

开发抗HER2抗体结合脂质液体纳米囊 (LLNCs-αHER2).
在实验室中使用胎儿牛血清和人类纤维素原的蛋白质冠状形成.
使用动态光散射 (DLS),纳米粒子跟踪分析 (NTA),激光多普勒电泳 (LDE) 和凝染色学进行物理化学表征.
使用SKBR3 (HER2-阳性) 和HDFa (HER2-阴性) 细胞系进行细胞吸收研究.

主要成果:

与HDFa细胞相比,LLNCs-αHER2在SKBR3细胞中的运输速率高出50倍.
蛋白冠状体 (LLNCs-αHER2-PC) 的存在显著降低了SKBR3细胞中40%的细胞吸收.
物理化学表征证实了纳米载体上蛋白质冠状体的形成.

结论:

在LLNCs上蛋白冠状形成不会阻碍特定细胞向HER2表达细胞.
冠状蛋白显著调节目标纳米载体的细胞吸收效率.
这些发现凸显了在用于癌症治疗的纳米医药设计中考虑蛋白质冠状病毒效应的重要性.

关键词:

积极的目标化是积极的目标化.乳腺癌乳腺癌乳腺癌细胞吸收细胞吸收.脂质液体纳米囊脂质液体纳米囊蛋白质冠状病毒冠状病毒

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