软体动物中MafF_G_K的功能意义:对Nrf2-介导的氧化应激反应的影响
Ronghui Yao1, Longmei Qiu1, Li Zhu1
1National Engineering Research Center of Marine Facilities Aquaculture, Marine Science and Technology College, Zhejiang Ocean University, Zhoushan 316004, China.
International journal of molecular sciences
|December 9, 2023
概括
这项研究确定了一种新的小Maf蛋白,McMafF_G_K,在中与Nrf2相互作用,在调节对环境压力的抗氧化反应中发挥关键作用.
科学领域:
- 海洋生物学 海洋生物学
- 分子毒理学分子毒理学
- 环境科学环境科学
背景情况:
- 核因子红色素2相关因子2 (Nrf2) 对于哺乳动物的抗氧化基因表达至关重要.
- 在软体动物中,特别是像Mytilus coruscus这样的海洋双动物中,Nrf2通路的机制尚不清楚.
- 已知小Maf蛋白通过其BZip域与Nrf2异构.
研究的目的:
- 为了研究一个小的Maf同类 (McMafF_G_K) 在海Mytilus coruscus中的作用.
- 阐明McMafF_G_K在Nrf2信号通路中的参与及其对环境压力因素的反应.
- 评估McMafF_G_K和McNrf2在氧化应激防御中的功能相互作用.
主要方法:
- 在Mytilus coruscus的McMafF_G_K基因的克隆中.
- 对McMafF_G_K表达的转录分析,以应对[a]pyrene (Bap) 暴露和siRNA干扰.
- 活性氧物种 (ROS) 和总抗氧化能力 (T-AOC) 的体外分析.
- 谷氨S转移酶 (GST) 下拉试验以确认蛋白质-蛋白质相互作用.
主要成果:
- 确定了McMafF_G_K,并证明它具有BZip域,这表明它在Nrf2通路中的作用.
- 麦克马夫F_G_K表达被Bap诱导,但被siRNA抑制,与Nrf2,NQO-1和GPx正相关.
- 巴普暴露增加了ROS和T-AOC,而McMafF_G_K倒置增加了ROS和降低了T-AOC.
- GST下拉试验证实了McMafF_G_K和McNrf2.2之间的物理相互作用.
结论:
- 麦克马夫F_G_K是Mytilus coruscus中的一个功能性小Maf蛋白,参与Nrf2介导的抗氧化反应.
- 麦克马夫GK在缓解环境污染物如Bap引起的氧化压力方面发挥着重要作用.
- 这项研究提供了对海洋软体生物氧化应激反应的分子机制的见解,并将McMafF_G_K确定为潜在的研究目标.
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