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植物中原生质和短暂表达系统的隔离,净化和应用

Kebin Chen^1,2, Jiali Chen^1,2, Xin Pi¹

¹Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, Changsha 410004, China.

International journal of molecular sciences

|December 9, 2023

概括

植物原生质,多功能植物细胞衍生物,是基因编辑和单细胞测序等应用的关键,因为它们的全能性和基因传递效率. 本综述详细介绍了植物科学中的它们的隔离,活力和短暂表达系统.

科学领域:

植物生物技术植物生物技术
细胞生物学细胞生物学
分子生物学分子生物学

背景情况:

原生质,缺乏细胞壁的植物细胞,具有全能性.
它们是各种生物和生物技术应用中的关键工具.
它们的实用性通过可访问性和高效的外源基因整合来增强.

研究的目的:

为植物科学中原始塑料应用提供全面的概述.
详细介绍原生质隔离,净化和生存能力评估方法.
探索原生质细胞短暂表达系统的建立和利用.

主要方法:

审查关于原生质隔离和应用的当前文献.
分析影响原生质隔离和活力的因素.
检查原生细胞短暂表达系统的方法.

主要成果:

原生质使各种应用成为可能,包括蛋白质定位,基因调节,功能研究,基因编辑和单细胞测序.
有效的隔离和可行性评估对于成功的原始塑料应用至关重要.
短暂表达系统为研究植物基因功能提供了强大的方法.

结论:

原塑体是现代植物科学中不可或缺的工具,促进了先进的研究和生物技术的发展.
优化隔离和短暂表达协议对于最大限度地提高各种植物物种中的原生质实用性至关重要.
本综述是研究人员利用原生质在植物科学中的一个有价值的资源.

关键词:

申请申请表申请表申请表基因表达的基因表达方式植物植物植物植物植物植物.原始塑料是原始塑料中的一个.过渡的转化转化是暂时的.

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