评估八种广泛使用的抗生素对河流微生物群落的生态毒性
María Rosa Pino-Otín1, Guillermo Lorca1, Elisa Langa1
1Faculty of Health Sciences, San Jorge University, 50830 Zaragoza, Spain.
International journal of molecular sciences
|December 9, 2023
概括
河流中的抗生素残留物会影响微生物群落. 高度会抑制生长和新陈代谢,而低度会产生多种影响,可能破坏水生生态系统.
科学领域:
- 环境科学 环境科学
- 微生物学 微生物学
- 生态毒理学 生态毒理学
背景情况:
- 抗生素残留物 (ABX) 在全球河流中普遍存在,需要进行生态毒理学评估.
- 河流微生物群落是环境压力的敏感生物指标.
- 了解ABX对这些社区的影响对于水生生态系统健康至关重要.
研究的目的:
- 量化八种常见抗生素对淡水河流微生物群体的影响.
- 评估微生物生长和生理特征的变化,以应对抗生素暴露.
- 描述河流微生物群落结构及其对抗生素压力的反应.
主要方法:
- 使用Biolog EcoPlatesTM来测量微生物生长和代谢活动.
- 采用16S rRNA基因测序用于微生物社区的特征.
- 在高 (100微克/毫升) 和低 (1微克/毫升) 度下测试了抗生素作用.
主要成果:
- 所有测试的抗生素在100μg/mL显著减少微生物生长和代谢能力,特别是聚合物,碳水化合物和酸.
- 氨基醇,红红素和 gentamicin 显示出最高的毒性.
- 在1μg/mL时,一些抗生素增强了新陈代谢,除了阿莫西西林,它在所有基质上都降低了新陈代谢.
结论:
- 虽然在河流检测到的ng/L度下,急性毒性不太可能发生,但长期暴露可能会破坏河流微生物群落.
- 使用多样化的微生物群落可以比单个物种研究更准确地评估抗生素的影响.
- 抗生素可以改变河流微生物群落的代谢功能,对生态系统服务产生影响.
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