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研究地面常春藤 (Glechoma hederacea L.) 提取物的功能潜力,使用体外方法
Danijela Šeremet1, Ksenija Durgo1, Jelena Kosanović1
1Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10 000 Zagreb, Croatia.
International journal of molecular sciences
|December 9, 2023
概括
地上的常春藤提取物显示出显著的抗氧化和抗微生物特性. 它保护了等离子体DNA和蛋白质免受损伤,并抑制了E. coli和S. aureus等致病细菌.
科学领域:
- 植物化学 植物化学
- 药理学 药理学是指药理学的学科.
- 细胞生物学 细胞生物学
背景情况:
- 地 (Glechoma hederacea L.) 是一种传统的药用草本.
- 它的生物活性多化合物具有抗氧化和抗微生物活性.
- 这些特性表明,有潜力治疗与氧化应激相关的细菌感染和慢性疾病.
研究的目的:
- 为了评估地面常春藤提取物在人类癌症细胞系 (肝脏,舌头,胃,结肠) 上的生物活性.
- 用细胞巨分子评估其抗氧化能力.
- 确定其对人类微生物群和致病细菌的影响.
主要方法:
- 细胞毒性,反应性氧物种的形成,以及对人类细胞系的基因毒性测定.
- 用蛋白质和DNA模型评估抗氧化能力.
- 对L. plantarum,E. coli和S. aureus进行抗菌活性测试.
主要成果:
- 细胞毒性因细胞类型而异;亲氧化作用随着暴露时间而减少.
- 最高度 (0.25 mg mL-1) 显示出显著的复生保护作用 (95.89%的等离子体DNA保存).
- 这种度还在蛋白质模型上表现出优异的抗氧化活性,以及对大肠杆菌和黄金杆菌的强烈抗菌作用.
结论:
- 地下常春藤提取物表现出度依赖的生物活动.
- 它显示出作为一种功能成分的显著潜力,用于对抗氧化应激和细菌感染.
- 对其治疗应用的进一步研究是有必要的.
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