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在炎症过程中,Mmp12在巨细胞中被翻译调节

Silvia Kuntschar¹, Giulia Cardamone¹, Kevin Klann²

¹Institute of Biochemistry I, Faculty of Medicine, Goethe University Frankfurt, 60590 Frankfurt, Germany.

International journal of molecular sciences

|December 9, 2023

概括

巨细胞使用翻译控制来控制炎症. 通过调节矩阵金属化酶12 (MMP12) 生产和巨细胞迁移,化细胞或清除死细胞有助于解决炎症.

科学领域:

免疫学免疫学免疫学
分子生物学分子生物学
细胞生物学细胞生物学

背景情况:

转录后调节对于适应性炎症反应至关重要.
在炎症解消过程中发生的转化性改变尚不清楚.
巨细胞 (Mφ) 在炎症及其解决过程中发挥关键作用.

研究的目的:

为了研究炎症解消阶段巨细胞的转化变化.
为了识别特定的翻译调节的蛋白质,参与了以细胞酶驱动的分辨率.
阐明矩阵金属酶12 (MMP12) 在炎症期间巨细胞迁移中的作用.

主要方法:

用LPS/IFNγ刺激的骨髓衍生巨细胞的转化变化的分析,并经历了细胞分裂.
总RNA测序以确认亲解析的转录签名.
量化 de novo 蛋白质合成以确定翻译性改变.
功能性试验评估MMP12对巨细胞迁移的影响.

主要成果:

细胞亡性细胞化 (efferocytosis) 在巨细胞中诱导了一种亲分辨签名.
尽管转录发生了轻微的变化,但在细胞和非细胞巨细胞之间观察到新的蛋白质合成的显著差异.
矩阵金属化酶12 (MMP12) 在早期炎症期间被翻译抑制,其翻译在解析过程中恢复.
减少MMP12的产生增强了巨细胞的矩阵依赖迁移.

结论:

翻译控制,而不仅仅是转录控制,在炎症期间显著影响巨细胞功能.
转化调节MMP12是控制巨细胞迁移的关键机制.
这种调节策略允许巨细胞在早期炎症期间迁移,并在解决过程中限制它,帮助有效的炎症反应和解决.

关键词:

在Mmp12中,Mmp12是什么?有效细胞细胞化.这是一种炎症炎症炎症炎症.巨细胞是一个巨细胞.解决方案的解决方案解决方案的解决方案.翻译翻译翻译翻译翻译翻译

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