相关实验视频

Updated: Jul 9, 2025

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

对比RNA-Seq分析揭示了PDX模型生成期间组织特定的剪接变异

Eun Ji Lee¹, Seung-Jae Noh², Huiseon Choi²

¹Department of Physiology, University of Ulsan College of Medicine, Asan Medical Center, Seoul 05505, Republic of Korea.

International journal of molecular sciences

|December 9, 2023

概括

患者衍生异种移植 (PDX) 显示保存的RNA生物型,但改变了拼接模式,特别是跳过的前子. 胃癌PDX模型显示逆基因相关性,影响瘤完整性.

科学领域:

在瘤学瘤学.
基因组学就是基因组学.
分子生物学分子生物学

背景情况:

组织特异性基因表达决定了器官功能和瘤特征.
了解患者衍生异体移植 (PDX) 中组织特异性如何保持对于癌症研究至关重要.
免疫功能受损的小鼠模型被广泛用于临床前癌症研究.

研究的目的:

为了比较原发性瘤及其相应的患者衍生异体移植 (PDX) 之间的基因表达和拼接模式.
在PDX模型中评估组织特异性基因表达的保存.
在PDX瘤的形成过程中识别RNA剪接的变化.

主要方法:

对四对原发性-PDX瘤对进行RNA测序 (RNA-seq) 的比较分析.
对RNA生物型分布和拼接模式的分析.
组织特定基因的相关性分析.
拼接变种特定的RT-PCR验证.

主要成果:

在初级瘤和PDX之间观察到保守的RNA生物型分布.
在PDX瘤中检测到拼接模式的显著变化,主要是跳过的外.
在初级和PDX对之间发现了组织特异性基因表达的强烈正相关性,胃癌除外.
胃癌PDX模型显示组织特异性基因表达的反相关性.

结论:

PDX形成可以导致剪接事件的组织特异性变化.
拼接变化代表了影响初级-PDX关系完整性的可变因素.
这些发现强调了在解释PDX模型数据时考虑拼接变化的重要性.

关键词:

这是一个PDX模型.在RNA-seqqq.替代性拼接是一种替代性的拼接.组织特异性组织特异性

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相关概念视频

RNA Splicing

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

Alternative RNA Splicing

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...