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PDGFRβ激活通过增强NFYA核定位诱导牛胚胎基因组激活

Chalani Dilshani Perera¹, Muhammad Idrees^1,2, Abdul Majid Khan¹

¹Division of Applied Life Science (BK21 Four), Gyeongsang National University, Jinju 52828, Republic of Korea.

International journal of molecular sciences

|December 9, 2023

概括

血小板衍生生增长因子受体-β (PDGFRβ) 信号通路调节核转录因子Y亚单元A (NFYA) 的表达,这对于牛胚胎基因组激活 (EGA) 和发育至关重要. 这个轴影响基因表达,胚胎发育和植入潜力.

科学领域:

生殖生物学生殖生物学
发展生物学发展生物学
分子生物学分子生物学

背景情况:

胚胎基因组激活 (EGA) 对于哺乳动物发育至关重要.
EGA的精确监管机制,特别是涉及转录因子,需要进一步阐明.
核转录因子Y亚单元A (NFYA) 是发育中的关键转录因子.

研究的目的:

研究NFYA在牛EGA和胚胎发育中的作用.
探索NFYA与牛胚胎中血小板衍生的生长因子受体-β (PDGFRβ) 信号传导之间的关系.
确定PDGFRβ调节对NFYA表达和随后的发育事件的影响.

主要方法:

使用PDGF-BB (激活剂) 和CP-673451 (抑制剂) 来调节牛胚胎中的PDGFRβ活性.
量化的NFYA和PDGFRβmRNA表达水平.
评估了EGA相关基因的表达, trofhoblast分化标记物 (CDX2),多能性标记物,活性氧物种 (ROS) 和线粒体亡水平.
评估了牛胚胎发育率和植入潜力.

主要成果:

PDGFRβ活性显著影响了NFYA表达.
PDGFRβmRNA水平由PDGF-BB上调,由CP-673451下调.
低调NFYA对EGA基因表达,胚胎发育率和植入潜力产生了负面影响.
NFYA下调影响了ROS,线粒体亡,多能性标记物和CDX2表达.

结论:

PDGFRβ-NFYA信号轴是牛胚胎基因组激活的关键调节器.
这种途径在早期的牛胚胎发育中起着至关重要的作用,影响基因表达,细胞分化和发育潜力.

关键词:

NFYAYA NFYAYA 的意思是这是PDGFRββ.激活器激活器是什么牛的胚胎细胞囊.胚胎基因组激活活动

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