细胞外矩阵沉积定义了从人类脂肪酸衍生的MSC细胞板组装的持续时间
Valentina S Glazieva1,2, Natalya A Alexandrushkina1,2, Peter P Nimiritsky1,2
1Institute for Regenerative Medicine, Medical Research and Education Center, Lomonosov Moscow State University, 27-10, Lomonosovskiy av., Moscow 119192, Russia.
International journal of molecular sciences
|December 9, 2023
概括
介酶体 stromal 细胞 (MSC) 板在较短的滞后阶段培养物中组装得更快. 在MSC中的高原I和IV含量与更长的组装时间相关,影响再生医学应用.
科学领域:
- 再生医学是一种再生医学.
- 细胞生物学 细胞生物学
- 生物材料工程 生物材料工程
背景情况:
- 细胞表 (CS) 工程与介质细胞 stromal 细胞 (MSCs) 是再生医学有前途的.
- 了解影响CS组装时间的因素对于临床应用至关重要.
研究的目的:
- 调查影响MSC衍生细胞片组装时间的因素.
- 确定优化CS生产用于治疗用途的关键参数.
主要方法:
- 在体外比较研究,使用来自14名健康捐赠者的脂肪衍生的MSC.
- 评估供体性别,MSC培养特征 (滞后阶段,翻倍时间) 和细胞外矩阵 (ECM) 组成.
- 对CS组件持续时间的定性和定量分析,包括原蛋白I,原蛋白IV,α-SMA和FAP-α.
主要成果:
- 较高的 I 原蛋白和 IV 原蛋白含量与较长的 CS 组装时间相关.
- 较短的MSC培养滞后阶段导致了更快的CS组装.
- 在可拆卸的CS中,FAP-α与组装时间负相关,这表明它在MSC激活和ECM周转方面发挥了作用.
- 年龄,性别,纤维素,拉敏素,α-SMA与组装时间没有显著相关.
结论:
- MSC培养滞后阶段和ECM组成,特别是原含量,是CS组装持续时间的关键决定因素.
- 结果为优化MSC基础的CS生产用于再生医学提供了洞察力.
- 对TGF-β1信号通路在ECM调节中的作用进行进一步研究是有必要的.
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