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人类带介质干细胞衍生的细胞外囊减少体外皮肤炎症

Tzou-Yien Lin¹, Tsong-Min Chang², Wei-Cheng Tsai³

¹Department of Paediatrics, Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Taoyuan 33305, Taiwan.

International journal of molecular sciences

|December 9, 2023

概括

人类带介质干细胞衍生的细胞外囊泡 (UCMSC-EVs) 保护皮肤细胞免受损伤. 这些EV能增强抗氧化防御,减少炎症,显示出抗衰老护肤产品的潜力.

科学领域:

细胞生物学细胞生物学
皮肤病学皮肤病学
再生医学是一种再生医学.

背景情况:

奥克萨佐隆诱导人体角质细胞 (HaCaT细胞) 的损伤,模仿皮肤损伤.
来自介细胞干细胞的细胞外囊泡 (EV) 正在研究其治疗潜力.
人类带介质干细胞 (UCMSC) 是强大的EVs的来源.

研究的目的:

调查UCMSC衍生的EVs在HaCaT细胞中抗氧化诱导的损伤的保护作用.
探索UCMSC-EVs的保护和抗炎作用的潜在机制.
评估UCMSC-EVs在抗衰老化品应用中的潜力.

主要方法:

在暴露于oxazolone之前,HaCaT细胞被UCMSC-EVs预处理.
测量了细胞活力,活性氧物种 (ROS) 水平和线粒体膜潜力.
分析了炎症性细胞因子 (IL-1β,TNF-α),SIRT1,P53和P65蛋白质的表达.

主要成果:

UCMSC-EV预处理显著改善了细胞活力,并减少了ROS和线粒体损伤.
通过降低IL-1β和TNF-α水平,UCMSC-EV显示出抗炎作用.
机制分析显示SIRT1和P53增加,P65蛋白表达减少.

结论:

源自UCMSC的EV具有保护性质,可以抵御oxazolone诱导的皮肤细胞损伤.
UCMSC-EVs增强细胞抗氧化剂防御系统,并表现出抗炎活性.
这些发现表明,UCMSC-EVs作为抗衰老护肤的功能成分具有前景.

关键词:

在 NF-κBB 中.一个SIRT1系统.细胞外囊泡细胞外囊泡在p53中,p53是什么?从带衍生出的介质细胞干细胞.

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