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酸性生长条件稳定了核糖体RNA基因集群,并通过非编码转录抑制延长了寿命

Yo Hasegawa^1,2, Hiroyuki Ooka^1,2, Tsuyoshi Wakatsuki^1,2,3

¹Laboratory of Genome Regeneration, Institute for Quantitative Biosciences (IQB), The University of Tokyo, Bunkyo-ku, Japan.

Genes to cells : devoted to molecular & cellular mechanisms

|December 9, 2023

概括

黑提取物稳定了核糖体RNA基因集群 (rDNA),并通过抑制非编码转录来延长酵母的寿命. 将生长介质的酸度调整为pH4.5可以增强这种效果,这种效果是由RPD3L基因组脱乙酶复合体介导的.

科学领域:

遗传学是一个遗传学.
分子生物学分子生物学
生物化学生化学

背景情况:

黑胡卜提取物 (BCE) 能够保护DNA免受各种物质的破坏.
核糖体RNA基因集群 (rDNA) 是一个高度不稳定的基因组区域.
基因组不稳定与衰老和癌症有关.

研究的目的:

为了研究黑提取物对rDNA稳定性和寿命的影响.
阐明 BCE. 通过稳定rDNA的基础分子机制.
探索生长媒介酸度在rDNA稳定性中的作用.

主要方法:

发芽酵母 (Saccharomyces cerevisiae) 的模型系统.
用黑提取物 (BCE) 和分成的BCE进行处理.
测量rDNA稳定性和染色体外循环 (ERC) 的形成.
对基因表达和基因脱乙酶活性的分析.
对生长媒介pH的操纵.

主要成果:

通过抑制基因间隔器 (IGS) 中的非编码转录,BCE稳定了rDNA并延长了酵母的寿命.
减少ERC的形成表明,rDNA稳定性得到了改善,而BCE.
将生长媒介的pH值降至4.5~,显著提高了rDNA稳定性和寿命.
涉及RPD3基因的RPD3L基因脱乙酶复合体,调解了pH依赖的稳定.

结论:

黑胡卜提取物通过调节rDNA促进基因组稳定性和寿命.
生长媒介的酸性是影响rDNA稳定的关键因素.
在保持rDNA完整性方面,RPD3L基因组脱乙酶复合体起着关键作用.
研究结果表明,在酸性微环境 (如炎症组织) 中对基因组维护有潜在的治疗意义.

关键词:

酸性酸性是指酸性的程度.黑是一种黑.基因组稳定性的基因组稳定性希斯顿脱乙酶的使用寿命长度寿命长度非编码转录的转录.这是一种核糖体RNA基因 (rDNA).衰老是一种老化.

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