在使用CRISPR-Cas9的Tamoxifen诱导型Tfap2b-CreERT2小鼠中产生Tfap2b-CreER

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Updated: Jul 9, 2025

Generation of Brown Fat-Specific Knockout Mice Using a Combined Cre-LoxP, CRISPR-Cas9, and Adeno-Associated Virus Single-Guide RNA System
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Generation of Brown Fat-Specific Knockout Mice Using a Combined Cre-LoxP, CRISPR-Cas9, and Adeno-Associated Virus Single-Guide RNA System

Published on: March 24, 2023

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Mingyi Zhang1, Jifan Feng1, Yue Li2

  • 1Center for Craniofacial Molecular Biology, University of Southern California, Los Angeles, California, USA.

Genesis (New York, N.Y. : 2000)
|December 9, 2023
PubMed
概括

我们开发了一个新的小鼠模型来追踪转录因子Tfap2b表达细胞. 该工具揭示了Tfap2b+细胞对面发育,四肢形成和牙发育的贡献.

关键词:
克里斯普尔-卡斯9是什么意思在Tfap2b-CreERT2中使用.鼠标的发展 鼠标的发展神经细胞是神经顶细胞.

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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
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