Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Electrospray Ionization (ESI) Mass Spectrometry01:12

Electrospray Ionization (ESI) Mass Spectrometry

867
Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
867

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Normalized peak distribution uniformity metrics for chromatographic evaluation.

Journal of chromatography. A·2026
Same author

A Protocol for Characterizing Comprehensive Two-Dimensional Liquid Chromatography Systems.

Journal of separation science·2026
Same author

Retention modeling of oligonucleotides on an amide-based HILIC column: A descriptor-driven approach.

Journal of chromatography. A·2026
Same author

Cannabis consumption is associated with altered steroid metabolism in young men.

Communications medicine·2026
Same author

Heterologous internal calibration for multiplexed internal quantification in targeted LC-MS/MS bioanalysis.

Journal of pharmaceutical and biomedical analysis·2026
Same author

Comprehensive evaluation of the analytical toolbox for commercial cysteine-linked antibody-drug conjugates.

Journal of chromatography. A·2026

相关实验视频

Updated: Jul 9, 2025

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS
07:34

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS

Published on: March 14, 2013

12.8K

基于ESI-MS的非向代谢学方面的挑战

Elena Tobolkina1,2, Víctor González-Ruiz3,2,4, Isabel Meister5,2

  • 1School of Pharmaceutical Sciences, University of Geneva, Geneva, Switzerland. Elena.Tobolkina@unige.ch.

Chimia
|December 9, 2023
PubMed
概括

本综述概述了液相分离方法与质谱学 (MS) 结合用于非目标代谢学. 它提供了关于生物样本数据采集,处理和解释的实际指导.

关键词:
标注注释 标注注释毛细管电泳可以通过毛细管电泳进行.液体色谱学 液体色谱学 液体色谱学质谱测量质量谱测量代谢学 代谢学 代谢学超临界流体色谱学 超临界流体色谱学毒理学和兴奋剂分析分析

更多相关视频

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS
11:00

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS

Published on: May 20, 2013

22.5K
A Strategy for Sensitive, Large Scale Quantitative Metabolomics
14:18

A Strategy for Sensitive, Large Scale Quantitative Metabolomics

Published on: May 27, 2014

21.0K

相关实验视频

Last Updated: Jul 9, 2025

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS
07:34

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS

Published on: March 14, 2013

12.8K
Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS
11:00

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS

Published on: May 20, 2013

22.5K
A Strategy for Sensitive, Large Scale Quantitative Metabolomics
14:18

A Strategy for Sensitive, Large Scale Quantitative Metabolomics

Published on: May 27, 2014

21.0K

科学领域:

  • 分析化学 分析化学
  • 生物化学 生物化学
  • 系统生物学 系统生物学

背景情况:

  • 非定位代谢学对于理解生物系统反应至关重要.
  • 液相分离技术与质谱学相结合,是全面的代谢分析的关键.

研究的目的:

  • 提供液相分离方法的概述,用于非目标代谢学.
  • 提供关于数据采集,处理和解释的实际指导.
  • 突出代谢学研究中常见的挑战和解决方案.

主要方法:

  • 液体染色学-质谱学 (LC-MS) 用于非目标代谢学.
  • 气色谱-质谱学 (GC-MS) 用于非目标代谢学.
  • 毛细电泳质谱法 (CE-MS) 用于非目标代谢学.

主要成果:

  • 详细审查各种液相分离技术,适用于非目标代谢学.
  • 讨论数据采集策略,以最大限度地覆盖代谢物.
  • 对信号处理,数据处理和生物解释的洞察力.

结论:

  • 液相分离是MS的一个强大的方法,用于非目标代谢.
  • 仔细考虑方法和数据处理对于可靠的结果至关重要.
  • 本综述是该领域研究人员的实用指南.